BRENDA - Enzyme Database
show all sequences of 1.14.11.8

Mammalian enzymes of trimethyllysine conversion to trimethylaminobutyrate

Henderson, L.M.; Nelson, P.J.; Henderson, L.; Fed. Proc. 41, 2843-2847 (1982)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
sparing as well as augmenting effect
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-N-Acetyltrimethyllysine
-
Bos taurus
citrate
-
Bos taurus
Hydroxytrimethyllysine
4 stereoisomers
Bos taurus
isocitrate
-
Bos taurus
Lactate
-
Bos taurus
malonate
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
succinate
-
Bos taurus
Zn2+
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
N6,N6,N6-Trimethyl-L-lysine
-
Bos taurus
0.22
-
alpha-ketoglutarate
-
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Bos taurus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
no stimulation
Bos taurus
Fe2+
preincubation with Fe2+ in absence of other cofactors inactivates (ascorbate or dithiothreitol prevents inactivation); required
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
65000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
180000
-
gel filtration
Bos taurus
Organism
Organism
UniProt
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
cortex (67%), medulla (33%)
Bos taurus
-
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
0°C, half-life: 5 days
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine
7020
Bos taurus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
half-life: 5 days
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
ascorbate
required
Bos taurus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
sparing as well as augmenting effect
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ascorbate
required
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-N-Acetyltrimethyllysine
-
Bos taurus
citrate
-
Bos taurus
Hydroxytrimethyllysine
4 stereoisomers
Bos taurus
isocitrate
-
Bos taurus
Lactate
-
Bos taurus
malonate
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
succinate
-
Bos taurus
Zn2+
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
N6,N6,N6-Trimethyl-L-lysine
-
Bos taurus
0.22
-
alpha-ketoglutarate
-
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Bos taurus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
no stimulation
Bos taurus
Fe2+
preincubation with Fe2+ in absence of other cofactors inactivates (ascorbate or dithiothreitol prevents inactivation); required
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
65000
-
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
180000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
cortex (67%), medulla (33%)
Bos taurus
-
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, half-life: 5 days
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
N6,N6, N6-trimethyl-L-lysine is epsilon-N-trimethyl-L-lysine
7020
Bos taurus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 52000 + x * 65000, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bos taurus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
half-life: 5 days
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Bos taurus
Other publictions for EC 1.14.11.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744681
Lesniak
Human carnitine biosynthesis ...
Homo sapiens
Chem. Commun. (Camb.)
53
440-442
2017
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
745958
Vijayendar Reddy
Fluorinated trimethyllysine a ...
Homo sapiens
Org. Biomol. Chem.
15
1350-1354
2017
1
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
745961
Reddy
Evidence that trimethyllysine ...
Homo sapiens
Org. Lett.
19
400-403
2017
-
-
-
-
-
-
-
-
1
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
744678
Al Temimi
Substrate scope for trimethyl ...
Homo sapiens
Chem. Commun. (Camb.)
52
12849-12852
2016
1
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
7
-
2
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
4
4
-
-
-
746366
Kazaks
Expression and purification o ...
Homo sapiens
Protein Expr. Purif.
104
1-6
2014
1
-
1
-
-
-
-
3
-
1
-
1
-
2
-
-
1
-
-
-
-
1
2
1
2
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
3
-
1
-
1
-
-
-
1
-
-
-
1
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
697027
Fischer
A moderate excess of dietary l ...
Sus scrofa
Br. J. Nutr.
101
190-196
2009
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
4
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
691166
Furusawa
Vitamin C is not essential for ...
Mus musculus
Biol. Pharm. Bull.
31
1673-1679
2008
1
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
686678
van Vlies
Submitochondrial localization ...
Rattus norvegicus
FEBS J.
274
5845-5851
2007
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671302
van Vlies
Measurement of carnitine biosy ...
Mus musculus, Rattus norvegicus
Anal. Biochem.
354
132-139
2006
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
8
-
-
2
-
6
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675029
Monfregola
Functional analysis of TMLH va ...
Homo sapiens, Mus musculus
J. Cell. Physiol.
204
839-847
2005
4
-
2
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
13
-
-
2
-
8
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
13
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675561
Davis
Carnitine deficiency and suppl ...
Rattus norvegicus
J. Nutr.
135
761-764
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
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-
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-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651370
Swiegers
Carnitine biosynthesis in neur ...
Neurospora crassa
FEMS Microbiol. Lett.
210
19-23
2002
-
-
-
-
-
-
-
2
-
1
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
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-
-
-
-
-
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2
-
1
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652164
Vaz
Molecular and biochemical char ...
Homo sapiens, Mus musculus, Rattus norvegicus
J. Biol. Chem.
276
33512-33517
2001
-
-
2
-
-
-
-
2
1
1
2
1
-
7
-
1
1
-
-
-
1
-
3
1
2
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
1
1
2
1
-
-
1
1
-
-
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
7020
Henderson
Mammalian enzymes of trimethyl ...
Bos taurus
Fed. Proc.
41
2843-2847
1982
1
-
-
-
-
-
9
2
1
2
3
-
-
1
-
-
1
-
-
2
1
1
1
1
-
1
-
1
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
9
-
2
1
2
3
-
-
-
-
1
-
2
1
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
7021
Sachan
Carnitine biosynthesis. Hydrox ...
Rattus norvegicus
Biochem. J.
188
529-534
1980
-
-
-
-
-
-
3
1
1
2
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
1
1
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7022
Sachan
Synthesis of carnitine from ep ...
Neurospora crassa
Biochem. Biophys. Res. Commun.
96
870-875
1980
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7019
Hulse
Carnitine biosynthesis. beta-H ...
Rattus norvegicus
J. Biol. Chem.
253
1654-1659
1978
-
-
-
-
-
-
-
-
2
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-