BRENDA - Enzyme Database show
show all sequences of 1.14.11.8

Molecular and biochemical characterization of rat epsilon-N-trimethyllysine hydroxylase, the first enzyme of carnitine biosynthesis

Vaz, F.M.; Ofman, R.; Westinga, K.; Back, J.W.; Wanders, R.J.A.; J. Biol. Chem. 276, 33512-33517 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
Homo sapiens
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.109
-
2-oxoglutarate
-
Rattus norvegicus
1.1
-
N6,N6,N6-Trimethyl-L-lysine
pH 6.7, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
exclusively in
Rattus norvegicus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
Km: 0.054 mM
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
2 * 43000, calculation from nucleotide sequence
Rattus norvegicus
87000
-
gel filtration, non-denaturing PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
Rattus norvegicus
first enzyme of the L-carnitine biosynthesis
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9NVH6
-
-
Mus musculus
Q91ZE0
-
-
Rattus norvegicus
Q91ZW6
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
the enzyme is synthesized as a 47500 Da precursor and processed to a mature protein of 43000 Da, presumably upon import into mitochondria
Rattus norvegicus
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0586
-
-
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
652164
Homo sapiens
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Homo sapiens
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
652164
Rattus norvegicus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Rattus norvegicus
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
first enzyme of the L-carnitine biosynthesis
652164
Rattus norvegicus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Rattus norvegicus
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 43000, calculation from nucleotide sequence
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
at 37°C
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
Homo sapiens
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.109
-
2-oxoglutarate
-
Rattus norvegicus
1.1
-
N6,N6,N6-Trimethyl-L-lysine
pH 6.7, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
exclusively in
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
Km: 0.054 mM
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
2 * 43000, calculation from nucleotide sequence
Rattus norvegicus
87000
-
gel filtration, non-denaturing PAGE
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
Rattus norvegicus
first enzyme of the L-carnitine biosynthesis
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
Rattus norvegicus
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
the enzyme is synthesized as a 47500 Da precursor and processed to a mature protein of 43000 Da, presumably upon import into mitochondria
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0586
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
652164
Homo sapiens
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Homo sapiens
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
652164
Rattus norvegicus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Rattus norvegicus
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
first enzyme of the L-carnitine biosynthesis
652164
Rattus norvegicus
3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
652164
Rattus norvegicus
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 43000, calculation from nucleotide sequence
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
at 37°C
Rattus norvegicus
Other publictions for EC 1.14.11.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744681
Lesniak
Human carnitine biosynthesis ...
Homo sapiens
Chem. Commun. (Camb.)
53
440-442
2017
-
-
1
-
-
-
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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2
2
-
-
-
745958
Vijayendar Reddy
Fluorinated trimethyllysine a ...
Homo sapiens
Org. Biomol. Chem.
15
1350-1354
2017
1
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
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3
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1
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1
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1
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1
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1
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3
-
1
-
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1
-
-
-
-
2
2
-
-
-
745961
Reddy
Evidence that trimethyllysine ...
Homo sapiens
Org. Lett.
19
400-403
2017
-
-
-
-
-
-
-
-
1
1
-
1
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2
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2
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1
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1
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1
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1
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2
-
1
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1
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2
2
-
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-
744678
Al Temimi
Substrate scope for trimethyl ...
Homo sapiens
Chem. Commun. (Camb.)
52
12849-12852
2016
1
-
-
-
-
-
-
-
-
1
-
1
-
2
-
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-
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7
-
1
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1
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1
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1
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1
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7
-
1
-
-
-
1
-
-
-
-
4
4
-
-
-
746366
Kazaks
Expression and purification o ...
Homo sapiens
Protein Expr. Purif.
104
1-6
2014
1
-
1
-
-
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-
3
-
1
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1
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2
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1
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2
1
1
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1
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1
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1
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3
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1
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1
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1
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1
2
1
1
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1
-
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1
1
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697027
Fischer
A moderate excess of dietary l ...
Sus scrofa
Br. J. Nutr.
101
190-196
2009
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-
-
-
-
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-
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1
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1
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2
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4
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1
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1
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1
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4
-
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1
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-
-
-
-
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1
1
1
1
-
-
691166
Furusawa
Vitamin C is not essential for ...
Mus musculus
Biol. Pharm. Bull.
31
1673-1679
2008
1
-
-
-
1
-
-
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1
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1
-
2
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1
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1
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1
1
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-
686678
van Vlies
Submitochondrial localization ...
Rattus norvegicus
FEBS J.
274
5845-5851
2007
-
-
-
-
-
-
-
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1
-
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1
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1
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1
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1
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1
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1
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671302
van Vlies
Measurement of carnitine biosy ...
Mus musculus, Rattus norvegicus
Anal. Biochem.
354
132-139
2006
-
-
-
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2
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2
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2
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8
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2
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2
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2
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2
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2
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8
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2
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675029
Monfregola
Functional analysis of TMLH va ...
Homo sapiens, Mus musculus
J. Cell. Physiol.
204
839-847
2005
4
-
2
-
2
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2
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3
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13
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2
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4
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2
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2
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2
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13
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2
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675561
Davis
Carnitine deficiency and suppl ...
Rattus norvegicus
J. Nutr.
135
761-764
2005
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2
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2
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1
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651370
Swiegers
Carnitine biosynthesis in neur ...
Neurospora crassa
FEMS Microbiol. Lett.
210
19-23
2002
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-
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2
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1
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3
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652164
Vaz
Molecular and biochemical char ...
Homo sapiens, Mus musculus, Rattus norvegicus
J. Biol. Chem.
276
33512-33517
2001
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2
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2
1
1
2
1
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7
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1
1
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1
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1
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1
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1
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1
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7020
Henderson
Mammalian enzymes of trimethyl ...
Bos taurus
Fed. Proc.
41
2843-2847
1982
1
-
-
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9
2
1
2
3
-
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1
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1
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2
1
1
1
1
1
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1
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1
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1
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1
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9
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2
1
2
3
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1
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2
1
1
1
1
1
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1
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1
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7021
Sachan
Carnitine biosynthesis. Hydrox ...
Rattus norvegicus
Biochem. J.
188
529-534
1980
-
-
-
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3
1
1
2
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1
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1
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1
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1
1
2
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1
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1
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7022
Sachan
Synthesis of carnitine from ep ...
Neurospora crassa
Biochem. Biophys. Res. Commun.
96
870-875
1980
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1
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1
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7019
Hulse
Carnitine biosynthesis. beta-H ...
Rattus norvegicus
J. Biol. Chem.
253
1654-1659
1978
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