Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pantoea ananatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Pantoea ananatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Pantoea ananatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | Pantoea ananatis | - |
(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2 | - |
? | |
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | Pantoea ananatis AJ13355 | - |
(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2 | - |
? | |
additional information | Pantoea ananatis | L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase | ? | - |
? | |
additional information | Pantoea ananatis AJ13355 | L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea ananatis | - |
- |
- |
Pantoea ananatis AJ13355 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | - |
Pantoea ananatis | (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2 | - |
? | |
(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2 | - |
Pantoea ananatis AJ13355 | (3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2 | - |
? | |
additional information | L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase | Pantoea ananatis | ? | - |
? | |
additional information | L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase | Pantoea ananatis AJ13355 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Pantoea ananatis |
Synonyms | Comment | Organism |
---|---|---|
HilB | - |
Pantoea ananatis |
L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating) | - |
Pantoea ananatis |
L-isoleucine-4'-dioxygenase | - |
Pantoea ananatis |