Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a rph1DELTA deletion mutant, phenotype overview | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for catalysis | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6,N6-trimethyl-L-lysine36 + 2-oxoglutarate + O2 | Saccharomyces cerevisiae | - |
histone H3 N6,N6-dimethyl-L-lysine36 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine36 + 2-oxoglutarate + O2 | Saccharomyces cerevisiae | - |
histone H3 N6-methyl-L-lysine36 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P39956 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 N6,N6,N6-trimethyl-L-lysine36 + 2-oxoglutarate + O2 | - |
Saccharomyces cerevisiae | histone H3 N6,N6-dimethyl-L-lysine36 + succinate + formaldehyde + CO2 | - |
? | |
histone H3 N6,N6-dimethyl-L-lysine36 + 2-oxoglutarate + O2 | - |
Saccharomyces cerevisiae | histone H3 N6-methyl-L-lysine36 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
JmjC demethylase | - |
Saccharomyces cerevisiae |
Rph1 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | changes in RENT component recruitment at NTS regions due to loss of H3 methylases or demethylases | Saccharomyces cerevisiae |
metabolism | different roles of histone H3 methylases in regulating Net1/Sir2 recruitment to rDNA regions and the resultant rDNA silencing. In particular, both H3K4 and H3K79 methylation by Set1 and Dot1 positively regulate rDNA silencing, whereas H3K36 methylation by Set2 has the opposite effect | Saccharomyces cerevisiae |
physiological function | changes in histone H3 lysine methylation levels distinctly regulate rDNA silencing by recruiting different silencing proteins to rDNA, thereby contributing to rDNA silencing and nucleolar organization in yeast. The Rph1/Kdm4 demethylase is a JHDM3/JMJD2 orthologue and has in vivo demethylase activity toward H3K36me3/2. Enzyme Rph1 positively affects transcription | Saccharomyces cerevisiae |
physiological function | Rph1 and Gis1 are reported to regulate the expression of PHR1, a photolyase gene required for the light-dependent repair of pyrimidine dimers. Both demethylases contain two zinc fingers and are damage responsive repressors of PHR1. Rph1 positively affects transcription | Saccharomyces cerevisiae |