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Literature summary for 1.14.11.67 extracted from

  • Ryu, H.; Ahn, S.
    Yeast histone H3 lysine 4 demethylase Jhd2 regulates mitotic ribosomal DNA condensation (2014), BMC Biol., 12, 75 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.67

Cloned(Commentary)

Cloned (Comment) Organism
gene JHD2, recombinant expression of HA-tagged wild-type Jhd2 and H427A mutant in yeast cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
H427A catalytically inactive mutant Saccharomyces cerevisiae
additional information generation of an enzyme mutant jhd2DELTA, jhd2DELTA profoundly enhances rDNA silencing. Overexpression of wild-type Jhd2 significantly reduces H3K4me3 levels, whereas overexpression of jhd2-H427A has little effect on H3K4me3 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 Saccharomyces cerevisiae yeast Jhd2 demethylates histone H3 Lys4 near the rDNA locus, Jhd2 demethylates H3K4 within the rDNA regions in vivo histone H3 N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P47156
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3 N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 yeast Jhd2 demethylates histone H3 Lys4 near the rDNA locus, Jhd2 demethylates H3K4 within the rDNA regions in vivo Saccharomyces cerevisiae histone H3 N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2
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 ?
histone H3 N6,N6,N6-trimethyl-L-lysine4 + 2-oxoglutarate + O2 yeast Jhd2 demethylates histone H3 Lys4 near the rDNA locus Saccharomyces cerevisiae histone H3 N6,N6-dimethyl-L-lysine4 + succinate + formaldehyde + CO2
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 ?

Synonyms

Synonyms Comment Organism
histone H3 lysine 4 demethylase
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 Saccharomyces cerevisiae
Jhd2
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 Saccharomyces cerevisiae

General Information

General Information Comment Organism
evolution Jhd2 is an evolutionarily conserved JARID1 family H3 Lys4 demethylase and a Jumonji C (JmjC) domain-containing histone demethylase (JHDM). Jhd2/Kdm5 is an evolutionarily conserved JARID1 family protein that has demethylase activity toward H3K4me3 in vitro or in vivo Saccharomyces cerevisiae
malfunction changes in RENT component recruitment at NTS regions due to loss of H3 methylases or demethylases. JHD2-deficient cells contain the mostly hypercondensed rDNA mislocalized away from the nuclear periphery Saccharomyces cerevisiae
metabolism different roles of histone H3 methylases in regulating Net1/Sir2 recruitment to rDNA regions and the resultant rDNA silencing. In particular, both H3K4 and H3K79 methylation by Set1 and Dot1 positively regulate rDNA silencing, whereas H3K36 methylation by Set2 has the opposite effect Saccharomyces cerevisiae
physiological function changes in histone H3 lysine methylation levels distinctly regulate rDNA silencing by recruiting different silencing proteins to rDNA, thereby contributing to rDNA silencing and nucleolar organization in yeast. Jhd2 regulates rDNA recombination through the Tof2/Csm1/Lrs4 pathway and prevents excessive recruitment of Tof2, Csm1/Lrs4 and condensin subunits to the replication fork barrier site within the NTS1 region. The demethylase activity of Jhd2 regulates telomeric silencing and mitotic rDNA condensation and rDNA repeat stability and rDNA silencing in a Sir2-independent manner by maintaining Csm1/Lrs4 and condensin association with rDNA regions during mitosis. Jhd2 is the only JmjC demethylase that contributes to the regulation of rDNA silencing and acts through a pathway that is independent of Net1 and Sir2, but through a Tof2/Csm1/Lrs4 pathway. Jhd2-mediated alleviation of excessive Csm1/Lrs4 or condensin at the NTS1 region of rDNA is required for the integrity of rDNA repeats and proper rDNA silencing during mitosis. The role of yeast JmjC-demethylases in regulating telomeric silencing is not restricted to Jhd2. The effect of Jhd2 on rDNA silencing is independent on the enzymatic activity of Jhd2 Saccharomyces cerevisiae