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Literature summary for 1.14.11.65 extracted from

  • Luka, Z.; Moss, F.; Loukachevitch, L.V.; Bornhop, D.J.; Wagner, C.
    Histone demethylase LSD1 is a folate-binding protein (2011), Biochemistry, 50, 4750-4756 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.65

Activating Compound

Activating Compound Comment Organism Structure
tetrahydrofolate binding kinetic analysis of folate derivatives, e.g. folates bound to glutamates, the (6R,S) form of the natural pentaglutamate form of tetrahydrofolate bound with the highest affinity to full-length LSD1, binding of different forms of folate to LSD1, and binding structures, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli BL21(DE3), recombinant expression of N-terminally truncated GST-tagged LSD1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus LSD1 is associated with folate Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 about, recombinant enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60341
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell nuclear extracts of HeLa cells contain LSD1 that is associated with folate Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the bifunctional enzyme catalyzes the demethylation of H3K9me2/me1 and H3K4me2/me1 (1.14.99.66) Homo sapiens ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 95059, LSD1 without N-terminal methionine, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
LSD1
-
 Homo sapiens
More see also for EC 1.14.99.66 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.5 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD required, a flavin-containing enzyme Homo sapiens

General Information

General Information Comment Organism
additional information mechanism of histone H3 demethylation by demethylase LSD1, overview Homo sapiens
physiological function methylation on the N-terminal tails of histone lysines serves as an epigenetic control mechanism, which is regulated by demathylases Homo sapiens