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Literature summary for 1.14.11.59 extracted from

  • Jonczyk, R.; Schmidt, H.; Osterrieder, A.; Fiesselmann, A.; Schullehner, K.; Haslbeck, M.; Sicker, D.; Hofmann, D.; Yalpani, N.; Simmons, C.; Frey, M.; Gier, A.
    Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: Characterization of Bx6 and Bx7 (2008), Plant Physiol., 146, 1053-1063.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 2-oxoglutarate in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Zea mays
0.373
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Zea mays 5737
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling highest expression Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + 2-oxoglutarate + O2
-
 Zea mays (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + succinate + CO2 + H2O
-
 ?
additional information 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one is not hydroxylated by the enzyme Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
benzoxazinless6
-
 Zea mays
BX6
-
 Zea mays
DIBOA-Glc dioxygenase
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.6
-
 (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside in 100 mM potassium phosphate buffer (pH 7.0), at 30°C Zea mays