BRENDA - Enzyme Database
show all sequences of 1.14.11.56

Microbial production of N-acetyl cis-4-hydroxy-L-proline by coexpression of the Rhizobium L-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1

Bach, T.M.; Hara, R.; Kino, K.; Ohtsu, I.; Yoshida, N.; Takagi, H.; Appl. Microbiol. Biotechnol. 97, 247-257 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
coexpression of L-proline cis-4-hydroxylase and N-acetyltransferase Mpr1 from Saccharomyces cerevisiae converting cis-4-hydroxy-L-proline into N-acetyl cis-4-hydroxy-L-proline in Escherichia coli. M9 medium containing L-proline produces more N-acetyl cis-4-hydroxy-L-proline than LB medium containing L-proline. The addition of NaCl and L-ascorbate results in a 2fold increase in N-acetyl cis-4-hydroxy-L-proline production in the L-proline-containing M9 medium
Sinorhizobium meliloti
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Sinorhizobium meliloti
Organism
Organism
UniProt
Commentary
Textmining
Sinorhizobium meliloti
-
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
coexpression of L-proline cis-4-hydroxylase and N-acetyltransferase Mpr1 from Saccharomyces cerevisiae converting cis-4-hydroxy-L-proline into N-acetyl cis-4-hydroxy-L-proline in Escherichia coli. M9 medium containing L-proline produces more N-acetyl cis-4-hydroxy-L-proline than LB medium containing L-proline. The addition of NaCl and L-ascorbate results in a 2fold increase in N-acetyl cis-4-hydroxy-L-proline production in the L-proline-containing M9 medium
Sinorhizobium meliloti
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Sinorhizobium meliloti
Other publictions for EC 1.14.11.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739780
Koketsu
Refined regio- and stereoselec ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099, Mesorhizobium loti 1021
ACS Synth. Biol.
4
383-392
2015
-
-
2
2
9
-
-
-
-
2
-
-
-
7
-
-
-
-
-
-
4
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
9
-
-
-
-
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2
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743931
Koketsu
Refined regio- and stereosele ...
Sinorhizobium meliloti, Mesorhizobium loti
ACS Synth. Biol.
4
383-392
2015
-
-
2
1
8
-
-
-
-
3
-
4
-
4
-
-
1
2
-
-
-
-
4
-
4
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
8
-
-
-
-
-
-
3
-
4
-
-
-
1
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
6
6
-
-
-
726771
Bach
Microbial production of N-acet ...
Sinorhizobium meliloti
Appl. Microbiol. Biotechnol.
97
247-257
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
7
-
-
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-
-
-
-
-
-
-
-
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-
-
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1
1
-
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-
-
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-
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-
-
-
-
-
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-
-
-
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-
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696034
Hara
Characterization of novel 2-ox ...
Sinorhizobium meliloti, Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochem. Biophys. Res. Commun.
379
882-886
2009
-
-
-
-
-
-
15
4
-
2
-
3
-
4
-
-
-
-
-
-
2
-
3
-
2
2
-
-
2
2
-
-
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-
-
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-
-
-
-
-
-
-
15
-
4
-
2
-
3
-
-
-
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-
-
2
-
3
-
2
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-
2
2
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-
-
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-
2
2