BRENDA - Enzyme Database
show all sequences of 1.14.11.56

Characterization of novel 2-oxoglutarate dependent dioxygenases converting L-proline to cis-4-hydroxy-L-proline

Hara, R.; Kino, K.; Biochem. Biophys. Res. Commun. 379, 882-886 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2 mM, no residual activity
Mesorhizobium loti
1,10-phenanthroline
2 mM, no residual activity
Sinorhizobium meliloti
Co2+
2 mM, 3% residual activity
Mesorhizobium loti
Co2+
2 mM, 2% residual activity
Sinorhizobium meliloti
Cu2+
2 mM, 0.3% residual activity
Mesorhizobium loti
Cu2+
2 mM, 1% residual activity
Sinorhizobium meliloti
diethyldicarbonate
2 mM, no residual activity
Mesorhizobium loti
diethyldicarbonate
2 mM, no residual activity
Sinorhizobium meliloti
EDTA
2 mM, 2% residual activity
Mesorhizobium loti
EDTA
2 mM, 1% residual activity
Sinorhizobium meliloti
Fe3+
2 mM, 68% residual activity
Sinorhizobium meliloti
Ni2+
2 mM, 8% residual activity
Mesorhizobium loti
Ni2+
2 mM, 4% residual activity
Sinorhizobium meliloti
Zn2+
2 mM, 0.5% residual activity
Mesorhizobium loti
Zn2+
2 mM, 1% residual activity
Sinorhizobium meliloti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
2-oxoglutarate
pH 7.5, 25°C
Mesorhizobium loti
0.3
-
2-oxoglutarate
pH 7.5, 25°C
Sinorhizobium meliloti
0.54
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
0.54
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
presence of ferrous iron is required
Mesorhizobium loti
Iron
presence of ferrous iron is required
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-proline + 2-oxoglutarate + O2
Mesorhizobium loti
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
L-proline + 2-oxoglutarate + O2
Sinorhizobium meliloti
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
L-proline + 2-oxoglutarate + O2
Mesorhizobium loti MAFF303099
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mesorhizobium loti
Q989T9
-
-
Mesorhizobium loti MAFF303099
Q989T9
-
-
Sinorhizobium meliloti
Q92LF6
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
684
-
pH 7.5, 25°C
Mesorhizobium loti
707
-
pH 7.5, 25°C
Sinorhizobium meliloti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Mesorhizobium loti
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Sinorhizobium meliloti
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Mesorhizobium loti MAFF303099
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
mlr6283
-
Mesorhizobium loti
R03107
-
Sinorhizobium meliloti
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Mesorhizobium loti
25
-
-
Sinorhizobium meliloti
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
25
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Mesorhizobium loti
7
-
-
Sinorhizobium meliloti
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2 mM, no residual activity
Mesorhizobium loti
1,10-phenanthroline
2 mM, no residual activity
Sinorhizobium meliloti
Co2+
2 mM, 2% residual activity
Sinorhizobium meliloti
Co2+
2 mM, 3% residual activity
Mesorhizobium loti
Cu2+
2 mM, 0.3% residual activity
Mesorhizobium loti
Cu2+
2 mM, 1% residual activity
Sinorhizobium meliloti
diethyldicarbonate
2 mM, no residual activity
Mesorhizobium loti
diethyldicarbonate
2 mM, no residual activity
Sinorhizobium meliloti
EDTA
2 mM, 2% residual activity
Mesorhizobium loti
EDTA
2 mM, 1% residual activity
Sinorhizobium meliloti
Fe3+
2 mM, 68% residual activity
Sinorhizobium meliloti
Ni2+
2 mM, 8% residual activity
Mesorhizobium loti
Ni2+
2 mM, 4% residual activity
Sinorhizobium meliloti
Zn2+
2 mM, 0.5% residual activity
Mesorhizobium loti
Zn2+
2 mM, 1% residual activity
Sinorhizobium meliloti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
-
2-oxoglutarate
pH 7.5, 25°C
Mesorhizobium loti
0.3
-
2-oxoglutarate
pH 7.5, 25°C
Sinorhizobium meliloti
0.54
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
0.54
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
presence of ferrous iron is required
Mesorhizobium loti
Iron
presence of ferrous iron is required
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-proline + 2-oxoglutarate + O2
Mesorhizobium loti
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
L-proline + 2-oxoglutarate + O2
Sinorhizobium meliloti
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
L-proline + 2-oxoglutarate + O2
Mesorhizobium loti MAFF303099
presence of 2-oxoglutarate is strictly required
cis-4-hydroxy-L-proline + succinate + CO2
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
684
-
pH 7.5, 25°C
Mesorhizobium loti
707
-
pH 7.5, 25°C
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Mesorhizobium loti
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Sinorhizobium meliloti
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
presence of 2-oxoglutarate is strictly required
696034
Mesorhizobium loti MAFF303099
cis-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Mesorhizobium loti
25
-
-
Sinorhizobium meliloti
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
25
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Mesorhizobium loti
7
-
-
Sinorhizobium meliloti
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
44
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
46
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
44
-
L-proline
pH 7.5, 25°C
Mesorhizobium loti
46
-
L-proline
pH 7.5, 25°C
Sinorhizobium meliloti
Other publictions for EC 1.14.11.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739780
Koketsu
Refined regio- and stereoselec ...
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ACS Synth. Biol.
4
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2
2
9
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-
-
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2
-
-
-
7
-
-
-
-
-
-
4
-
8
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
9
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743931
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Refined regio- and stereosele ...
Sinorhizobium meliloti, Mesorhizobium loti
ACS Synth. Biol.
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2015
-
-
2
1
8
-
-
-
-
3
-
4
-
4
-
-
1
2
-
-
-
-
4
-
4
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
8
-
-
-
-
-
-
3
-
4
-
-
-
1
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
6
6
-
-
-
726771
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Sinorhizobium meliloti
Appl. Microbiol. Biotechnol.
97
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-
1
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696034
Hara
Characterization of novel 2-ox ...
Sinorhizobium meliloti, Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochem. Biophys. Res. Commun.
379
882-886
2009
-
-
-
-
-
-
15
4
-
2
-
3
-
4
-
-
-
-
-
-
2
-
3
-
2
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
4
-
2
-
3
-
-
-
-
-
-
2
-
3
-
2
-
-
2
2
-
-
-
-
-
-
-
2
2