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Literature summary for 1.14.11.53 extracted from

  • Xie, L.J.; Yang, X.T.; Wang, R.L.; Cheng, H.P.; Li, Z.Y.; Liu, L.; Mao, L.; Wang, M.; Cheng, L.
    Identification of flavin mononucleotide as a cell-active artificial N6-methyladenosine RNA demethylase (2019), Angew. Chem. Int. Ed. Engl., 58, 5028-5032 .
    View publication on PubMed
Search for more literature for 1.14.11.53

Application

Application Comment Organism
synthesis visible-light-induced oxidation of m6ARNA is performed in live cells under atmospheric O2 by applying the metabolite flavin mononucleotide (FMN) as an efficient photosensitizer synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AUUCUCAm6AC-3' + 2-oxoglutarate + O2 ssRNA substrate synthetic construct 5'-AUUCUCAAC-3' + formaldehyde + succinate + CO2
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 ?
5'-CUGGm6ACUGG-3' + 2-oxoglutarate + O2 ssRNA substrate synthetic construct 5'-CUGGACUGG-3' + formaldehyde + succinate + CO2
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5'-m6ACUGACUAG-3' + 2-oxoglutarate + O2 ssRNA substrate synthetic construct 5'-m6ACUGACUAG-3' + formaldehyde + succinate + CO2
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additional information poor substrates: N1-methyladenosine, O2'-methyladenosine, 5-methylcytosine, and 5-methyluridine synthetic construct ?
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N6,N6-dimethyladenosine + 2 2-oxoglutarate + 2 O2
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 synthetic construct adenosine + 2 formaldehyde + 2 succinate + 2 CO2
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N6-methyladenine + 2-oxoglutarate + O2
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 synthetic construct adenine + formaldehyde + succinate + CO2
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N6-methyladenine in RNA + 2-oxoglutarate + O2
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 synthetic construct adenine in RNA + formaldehyde + succinate + CO2
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N6-methyladenosine + 2-oxoglutarate + O2
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 synthetic construct adenosine + formaldehyde + succinate + CO2
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 ?

General Information

General Information Comment Organism
metabolism during the reaction, the hv-excited FMN abstracts one electron and one proton from N6-methyl in m6A, probably via a proton-coupled electron transfer pathway, to give the deprotonated amine radical cation and the semiquinone radical of FMNH. A second proton-coupled electron transfer then leads to the formation of the corresponding imine with the concomitant reduction of the FMNH radical to FMNH2. The N,O-hemiacetal hm6A is formed from the hydration of II[4a,b] and it quickly oxidizes to N6-formyladenosine, followed by water-assisted decomposition to yield the adenosine and formic acid. Alternatively, a direct loss of formaldehyde from N6-formyladenosine would also afford adenosine synthetic construct
physiological function flavin mononucleotide functions as an artificial m6A demethylase. FMN mediates substantial photochemical demethylation of m6A residues of RNA in live cells synthetic construct