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Literature summary for 1.14.11.53 extracted from
- Identification of specific N6-methyladenosine RNA demethylase FTO inhibitors by single-quantum-dot-based FRET nanosensors (2020), Anal. Chem., 92, 13936-13944 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a single-quantum-dot-based fluorescence resonance energy transfer (FRET) sensor for the identification of specific FTO demethylase inhibitors. The FTO-mediated demethylation of m6A can induce the cleavage of demethylated DNA to generate the biotinylated DNA fragments, which may function as capture probes to assemble the Cy5-labeled reporter probes onto the quantum dot surface, enabling the occurrence of FRET between the quantum dot and Cy5 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diacerein | not inhibitiory to isoform ALKBH5 | Homo sapiens | |
| additional information | not inhibitiory: rhein, diacerein | Homo sapiens | |
| Plumbagin | - |
Homo sapiens |  |
| Rhein | not inhibitiory to isoform ALKBH5 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6P6C2 | - |
- |
| Homo sapiens | Q9C0B1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin-AAGCTCCCATGTTAGGm6ATCAGTGTCTCGAG-biotin + 2-oxoglutarate + O2 | - |
Homo sapiens | biotin-AAGCTCCCATGTTAGGATCAGTGTCTCGAG-biotin + formaldehyde + succinate + CO2 | - |
? | |
| CATGTTAGGATCAGTG + 2-oxoglutarate + O2 | - |
Homo sapiens | CATGTTAGGm6ATCAGTG + formaldehyde + succinate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fat mass and obesity-associated enzyme | - |
Homo sapiens |
| FTO | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00109 | - |
pH 7.5, 30°C | Homo sapiens | Plumbagin | |
| 0.00151 | - |
pH 7.5, 30°C | Homo sapiens | diacerein | |
| 0.00163 | - |
pH 7.5, 30°C | Homo sapiens | Plumbagin | |
| 0.00166 | - |
pH 7.5, 30°C | Homo sapiens | Rhein | |
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Release 2023.1 (January 2023)
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