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Literature summary for 1.14.11.48 extracted from
- Purification and characterization of an FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae (2021), Protein Expr. Purif., 183, 105862 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aspergillus oryzae |
General Stability
| General Stability | Organism |
|---|---|
| protein is stabilized in solution in the presence of either 600 microM xanthine, 1 M NaCl, 600 microM 2-oxoglutarate or 20% glycerol | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | decrease of up to 46% in the relative activity in presence of 0.8 M NaCl | Aspergillus oryzae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40400 | - |
dynamic light scattering | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q2UP14 | - |
- |
| Aspergillus oryzae ATCC 42149 | Q2UP14 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, the nickel affinity purified protein is subjected to ion-exchange chromatography once the N-terminal 6XHis-tag has been successfully removed, followed by size-exclusion purification | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xanthine + 2-oxoglutarate + O2 | - |
Aspergillus oryzae | urate + succinate + CO2 | - |
? | |
| xanthine + 2-oxoglutarate + O2 | - |
Aspergillus oryzae ATCC 42149 | urate + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41720, calculated from sequence, 1 * 40000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AO090001000152 | - |
Aspergillus oryzae |
| TauD domain-containing protein | - |
Aspergillus oryzae |
| XanA | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
protein is stabilized in solution in the presence of either 600 microM xanthine, 1 M NaCl, 600 microM 2-oxoglutarate or 20% glycerol, which increases the melting temperature by 2, 4, 5 and 6 K, respectively | Aspergillus oryzae |
| 4 | 25 | stable within | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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