Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
C357A | enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Mutant enzyme is resistant rto thiol-specific reagents that inactivated wild-type enzyme. Elevated ferroxidase activity in the absence of substrates | Aspergillus nidulans |
D138A | enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine | Aspergillus nidulans |
D151A | inactive mutant enzyme | Aspergillus nidulans |
E137A | enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine | Aspergillus nidulans |
H149A | inactive mutant enzyme | Aspergillus nidulans |
H340A | mutant enzyme exhibits 0.17% of the wild-type enzyme activity | Aspergillus nidulans |
K122A | 2fold increase in the Kd of 2-oxoglutarate compared to wild-type enzyme | Aspergillus nidulans |
N358A | 23fold decrease in kcat/Km compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine | Aspergillus nidulans |
Q101A | elevated ferroxidase activity in the absence of substrates | Aspergillus nidulans |
Q356A | significant decrease in Ki(app) for 6,8-dihydroxypurine. Elevated ferroxidase activity in the absence of substrates | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6,8-dihydroxypurine | slow-binding competitive inhibitor | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
2-oxoglutarate | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
0.032 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
0.041 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
0.042 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
0.045 | - |
xanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
0.047 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
0.048 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
0.053 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
0.057 | - |
xanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
0.058 | - |
xanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
0.059 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
0.069 | - |
xanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
0.071 | - |
xanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
0.088 | - |
xanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
0.122 | - |
xanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
0.4 | - |
9-methylxanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
0.55 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
0.554 | - |
xanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
0.58 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
0.68 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
0.78 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
0.88 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
1 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
1.09 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | non-heme mononuclear Fe(II) enzyme | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q4QZZ9 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme variants are purified from recombinant Escherichia coli cells | Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-methylxanthine + 2-oxoglutarate + O2 | - |
Aspergillus nidulans | ? | - |
? | |
xanthine + 2-oxoglutarate + O2 | - |
Aspergillus nidulans | urate + succinate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus nidulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
1.26 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
1.4 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
1.9 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
2 | 8 | 2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
3 | 6 | xanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
3.8 | - |
9-methylxanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
4.25 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
4.5 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
5.9 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
17 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
19 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
21 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
25 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
31 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
32 | - |
xanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
34 | - |
xanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
35 | - |
xanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
38 | - |
xanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
40 | - |
xanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
42 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
60 | - |
xanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
67 | - |
2-oxoglutarate | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
72 | - |
xanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aspergillus nidulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
xanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
300 | - |
xanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
340 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
360 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
400 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
470 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
490 | - |
xanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
550 | - |
xanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
560 | - |
xanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
610 | - |
xanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
670 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
680 | - |
xanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
760 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
850 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q101A | Aspergillus nidulans | |
1260 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme N358A | Aspergillus nidulans | |
1310 | - |
2-oxoglutarate | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
1600 | - |
xanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
2050 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme Q356A | Aspergillus nidulans | |
2160 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme K122A | Aspergillus nidulans | |
2160 | - |
2-oxoglutarate | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans | |
5410 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme E137A | Aspergillus nidulans | |
7330 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme D138A | Aspergillus nidulans | |
8180 | - |
9-methylxanthine | pH 7.4, 25°C, mutant enzyme C357A | Aspergillus nidulans | |
9500 | - |
9-methylxanthine | pH 7.4, 25°C, wild-type enzyme | Aspergillus nidulans |