Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | - |
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis 2e2 | - |
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Bacillus thuringiensis 2e2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-allyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | S-allyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
(S)-allyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | S-allyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
(S)-ethyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | S-ethyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
(S)-ethyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | S-ethyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
(S)-methyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | S-methyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
(S)-methyl-L-cysteine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | S-methyl-L-cysteine (S)-sulfoxide + succinate + CO2 | - |
? | |
L-ethionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | L-ethionine (S)-sulfoxide + succinate + CO2 | - |
? | |
L-ethionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | L-ethionine (S)-sulfoxide + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis 2e2 | (2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-methionine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | L-methionine (S)-sulfoxide + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Bacillus thuringiensis |
isoleucine 4-hydroxylase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 28 | the enzyme retains over 80% of its activity after 1 h incubation at 20-28°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Bacillus thuringiensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the enzyme retains over 90% activity after 1 h of incubation at pH 7.0-9.0 | Bacillus thuringiensis |