BRENDA - Enzyme Database show
show all sequences of 1.14.11.43

Purification and characterization of two enantioselective alpha-ketoglutarate-dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans MH

Mller, T.A.; Fleischmann, T.; van der Meer, J.R.; Kohler, H.P.; Appl. Environ. Microbiol. 72, 4853-4861 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
slightly reduced in the absence of ascorbate
Sphingobium herbicidovorans
Cloned(Commentary)
Commentary
Organism
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS)
Sphingobium herbicidovorans
General Stability
General Stability
Organism
storage on ice and freeze-thaw cycles reduce the activity to about 80% of the original activity
Sphingobium herbicidovorans
Inhibitors
Inhibitors
Commentary
Organism
Structure
(S)-2-(2,4-dichlorophenoxy)propanoate
slight inhibition
Sphingobium herbicidovorans
(S)-2-(4-chloro-2-methylphenoxy)propanoate
slight inhibition
Sphingobium herbicidovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0196
-
2-oxoglutarate
pH 6.8, 30C
Sphingobium herbicidovorans
0.132
-
(S)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30C
Sphingobium herbicidovorans
0.159
-
O2
pH 6.8, 30C, above 0.23 mM
Sphingobium herbicidovorans
0.495
-
(S)-(2,4-dichlorophenoxy)propanoate
pH 6.8, 30C
Sphingobium herbicidovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
ferrous iron is necessary for activity of the enzyme and other divalent cations could not replace it
Sphingobium herbicidovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Sphingobium herbicidovorans
33700
-
1 * 33700, calculated from sequence
Sphingobium herbicidovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
Sphingobium herbicidovorans
i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
Sphingobium herbicidovorans
i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingobium herbicidovorans
Q700X4
-
-
Purification (Commentary)
Commentary
Organism
-
Sphingobium herbicidovorans
Storage Stability
Storage Stability
Organism
4C, 3 days, less than 10% loss of activity
Sphingobium herbicidovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
721337
Sphingobium herbicidovorans
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
721337
Sphingobium herbicidovorans
2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
-
-
?
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-dichlorprop. SdpA is specific for the (S) enantiomer
721337
Sphingobium herbicidovorans
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
-
?
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-mecoprop. SdpA is specific for the (S) enantiomer. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate
721337
Sphingobium herbicidovorans
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 32000, SDS-PAGE; 1 * 33700, calculated from sequence
Sphingobium herbicidovorans
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
40
-
Sphingobium herbicidovorans
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
45
20C: about 40% of maximal activity, 35-40C: optimal activity
Sphingobium herbicidovorans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
22
-
stable for several hours
Sphingobium herbicidovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sphingobium herbicidovorans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
pH 6.0: about 30% of maximal activity, pH 7.5: about 50% of maximal activity
Sphingobium herbicidovorans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
slightly reduced in the absence of ascorbate
Sphingobium herbicidovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as His6-tagged fusion protein from Escherichia coli BL21(DE3)(pLysS)
Sphingobium herbicidovorans
General Stability (protein specific)
General Stability
Organism
storage on ice and freeze-thaw cycles reduce the activity to about 80% of the original activity
Sphingobium herbicidovorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(S)-2-(2,4-dichlorophenoxy)propanoate
slight inhibition
Sphingobium herbicidovorans
(S)-2-(4-chloro-2-methylphenoxy)propanoate
slight inhibition
Sphingobium herbicidovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0196
-
2-oxoglutarate
pH 6.8, 30C
Sphingobium herbicidovorans
0.132
-
(S)-2-(4-chloro-2-methylphenoxy)propanoate
pH 6.8, 30C
Sphingobium herbicidovorans
0.159
-
O2
pH 6.8, 30C, above 0.23 mM
Sphingobium herbicidovorans
0.495
-
(S)-(2,4-dichlorophenoxy)propanoate
pH 6.8, 30C
Sphingobium herbicidovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
ferrous iron is necessary for activity of the enzyme and other divalent cations could not replace it
Sphingobium herbicidovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Sphingobium herbicidovorans
33700
-
1 * 33700, calculated from sequence
Sphingobium herbicidovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
Sphingobium herbicidovorans
i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
Sphingobium herbicidovorans
i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sphingobium herbicidovorans
Storage Stability (protein specific)
Storage Stability
Organism
4C, 3 days, less than 10% loss of activity
Sphingobium herbicidovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-dichlorprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
721337
Sphingobium herbicidovorans
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
-
?
(R)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-mecoprop, the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH. SdpA activity is completely absent in the absence of 2-oxoglutarate
721337
Sphingobium herbicidovorans
2-methyl-4-chlorophenol + pyruvate + succinate + CO2
-
-
-
?
(S)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-dichlorprop. SdpA is specific for the (S) enantiomer
721337
Sphingobium herbicidovorans
2,4-dichlorophenol + pyruvate + succinate + CO2
-
-
-
?
(S)-2-(4-chloro-2-methylphenoxy)propanoate + 2-oxoglutarate + O2
i.e. (S)-mecoprop. SdpA is specific for the (S) enantiomer. The enzyme is specific for 2-oxoglutarate. Low levels of activity (1.9%) in presence of 2-oxoadipate. No activity with oxaloacetate, 2-oxobutyrate, and 2-oxovalerate
721337
Sphingobium herbicidovorans
4-chloro-2-methylphenol + pyruvate + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 32000, SDS-PAGE; 1 * 33700, calculated from sequence
Sphingobium herbicidovorans
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
40
-
Sphingobium herbicidovorans
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
45
20C: about 40% of maximal activity, 35-40C: optimal activity
Sphingobium herbicidovorans
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
22
-
stable for several hours
Sphingobium herbicidovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sphingobium herbicidovorans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.5
pH 6.0: about 30% of maximal activity, pH 7.5: about 50% of maximal activity
Sphingobium herbicidovorans
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
Sphingobium herbicidovorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the degradation of phenoxyalkanoic acid herbicides in Sphingomonas herbicidovorans MH
Sphingobium herbicidovorans
Other publictions for EC 1.14.11.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721341
Paulin
Abundance and expression of en ...
Sphingobium herbicidovorans
Appl. Environ. Microbiol.
76
2873-2883
2010
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
721337
Mller
Purification and characterizat ...
Sphingobium herbicidovorans
Appl. Environ. Microbiol.
72
4853-4861
2006
1
-
1
-
-
1
2
4
-
1
2
2
-
3
-
-
1
-
-
-
-
1
4
1
1
1
1
-
1
1
-
-
-
-
-
1
-
1
-
-
-
1
-
2
-
4
-
1
2
2
-
-
-
1
-
-
-
1
4
1
1
1
1
-
1
1
-
-
-
1
1
-
-
-
723719
Mller
Structural basis for the enant ...
Sphingobium herbicidovorans
Protein Sci.
15
1356-1368
2006
-
-
-
-
9
-
-
5
-
-
-
-
-
1
-
-
1
-
-
-
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3
-
1
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5
1
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-
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-
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-
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9
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
3
-
1
-
-
5
1
-
-
-
-
-
-
-
5
5
723114
Westendorf
The two enantiospecific dichlo ...
Delftia acidovorans, Delftia acidovorans MC1
Microbiol. Res.
157
317-322
2002
-
-
-
-
-
-
-
-
-
-
2
4
-
4
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
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-
-
2
4
-
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1
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4
1
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1
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