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Literature summary for 1.14.11.4 extracted from

  • Rautavuoma, K.; Takaluoma, K.; Passoja, K.; Pirskanen, A.; Kvist, A.P.; Kivirikko, K.I.; Myllyharju, J.
    Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3: The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity (2002), J. Biol. Chem., 277, 23084-23091.
    View publication on PubMed
Search for more literature for 1.14.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells using a baculovirus vector Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-oxoglutarate lysyl hydroxylase 3 Homo sapiens
0.12
-
 2-oxoglutarate lysyl hydroxylase 1 Homo sapiens
0.35
-
 ascorbate lysyl hydroxylases 1 and 3 Homo sapiens
0.4
-
 (Ile-Lys-Gly)3 lysyl hydroxylase 1 Homo sapiens
0.43
-
 (L-Ile-L-Lys-Gly)3 lysyl hydroxylase 3 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, Km: 0.001-0.005 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 gel filtration, isoforms 1-3 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen + 2-oxoglutarate + O2 Homo sapiens enzyme required during collagen biosynthesis 5-hydroxylysyl-collagen + succinate + CO2
-
 ?
collagen + 2-oxoglutarate + O2 Homo sapiens influence the integrity and stability of collagen 5-hydroxylysyl-collagen + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 three isoenzymes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification hydroxylation of lysyl residue in -X-Lys-Gly Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
three recombinant isoenzymes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information existence of isoforms Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ile-Lys-Gly)3 + 2-oxoglutarate + O2
-
 Homo sapiens (Ile-5-hydroxylyseine-Gly)3 + succinate + CO2
-
 ?
2-oxoglutarate + O2 + ascorbate
-
 Homo sapiens succinate + CO2 + dehydroascorbate + H2O
-
 ?
collagen + 2-oxoglutarate + O2 enzyme required during collagen biosynthesis Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
 ?
collagen + 2-oxoglutarate + O2 influence the integrity and stability of collagen Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
 ?
peptidyl-L-lysine + 2-oxoglutarate + O2 synthetic peptides Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 80000-85000, SDS-PAGE, isoforms 1-3 Homo sapiens