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Literature summary for 1.14.11.4 extracted from
- Partial purification and characterization of protocollagen lysine hydroxylase from chick embryos (1972), Biochim. Biophys. Acta, 258, 366-379.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | - |
Gallus gallus | |
| catalase | - |
Gallus gallus | |
| dithiothreitol | - |
Gallus gallus |  |
General Stability
| General Stability | Organism |
|---|---|
| enzyme formes aggregates in low ionic strength buffer | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-mercuribenzoate | - |
Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
ascorbate | - |
Gallus gallus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, Km: 0.001-0.005 mM | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Gallus gallus |
| 550000 | - |
gel filtration, peak 2 | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + O2 + ascorbate | - |
Gallus gallus | succinate + CO2 + dehydroascorbate + H2O | - |
? | |
| peptidyl-L-lysine + 2-oxoglutarate + O2 | synthetic peptides | Gallus gallus | peptidyl-5-hydroxy-L-lysine + succinate + CO2 | hydroxy-L-lysine | ? | |
| peptidyl-L-lysine + 2-oxoglutarate + O2 | protocollagen | Gallus gallus | peptidyl-5-hydroxy-L-lysine + succinate + CO2 | hydroxy-L-lysine | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required | Gallus gallus | |
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