General Stability | Organism |
---|---|
loss of activity during concentration | Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Gallus gallus | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, Km: 0.001-0.005 mM | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two alternative procedures | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, enzyme purified by collagen-agarose column-chromatography stable, enzyme from Bio-gel column unstable | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidyl-L-lysine + 2-oxoglutarate + O2 | protocollagen | Gallus gallus | peptidyl-5-hydroxy-L-lysine + succinate + CO2 | hydroxy-L-lysine | ? | |
[procollagen]-L-lysine + 2-oxoglutarate + O2 | - |
Gallus gallus | [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 83000-85000, SDS-PAGE | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | 4.2 | [procollagen]-L-lysine | in synthetic peptides | Gallus gallus |