BRENDA - Enzyme Database
show all sequences of 1.14.11.39

An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics

Neary, J.M.; Powell, A.; Gordon, L.; Milne, C.; Flett, F.; Wilkinson, B.; Smith, C.P.; Micklefield, J.; Microbiology 153, 768-776 (2007)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-asparagine + 2-oxoglutarate + O2
Streptomyces coelicolor
-
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
?
L-asparagine + 2-oxoglutarate + O2
Streptomyces coelicolor A3(2)
-
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces coelicolor
Q9Z4Z5
-
-
Streptomyces coelicolor A3(2)
Q9Z4Z5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-asparagine + 2-oxoglutarate + O2
-
725895
Streptomyces coelicolor
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
-
?
L-asparagine + 2-oxoglutarate + O2
-
725895
Streptomyces coelicolor A3(2)
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
AsnO
-
Streptomyces coelicolor
asparagine oxygenase
-
Streptomyces coelicolor
SCO3236
-
Streptomyces coelicolor
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-asparagine + 2-oxoglutarate + O2
Streptomyces coelicolor
-
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
?
L-asparagine + 2-oxoglutarate + O2
Streptomyces coelicolor A3(2)
-
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-asparagine + 2-oxoglutarate + O2
-
725895
Streptomyces coelicolor
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
-
?
L-asparagine + 2-oxoglutarate + O2
-
725895
Streptomyces coelicolor A3(2)
(2S,3S)-3-hydroxyasparagine + succinate + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
deletion mutants produce several new Asn-containing calcium-dependent antibiotics not present in the wild type, which retain calcium-dependent antimicrobial activity
Streptomyces coelicolor
physiological function
the enzyme is required for the beta-hydroxylation of the Asn residue within calcium-dependent antibiotic
Streptomyces coelicolor
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion mutants produce several new Asn-containing calcium-dependent antibiotics not present in the wild type, which retain calcium-dependent antimicrobial activity
Streptomyces coelicolor
physiological function
the enzyme is required for the beta-hydroxylation of the Asn residue within calcium-dependent antibiotic
Streptomyces coelicolor
Other publictions for EC 1.14.11.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721127
Strieker
Mechanistic and structural bas ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
ACS Chem. Biol.
2
187-196
2007
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725895
Neary
An asparagine oxygenase (AsnO) ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Microbiology
153
768-776
2007
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