BRENDA - Enzyme Database show
show all sequences of 1.14.11.38

FtmOx1, a non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus

Steffan, N.; Grundmann, A.; Afiyatullov, S.; Ruan, H.; Li, S.M.; Org. Biomol. Chem. 7, 4082-4087 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Aspergillus fumigatus
Cloned(Commentary)
Commentary
Organism
-
Aspergillus fumigatus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM
Aspergillus fumigatus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Aspergillus fumigatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35100
-
2 * 35100, calculated from sequence
Aspergillus fumigatus
38000
-
2 * 38000, SDS-PAGE
Aspergillus fumigatus
81000
-
gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
Aspergillus fumigatus
the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A
verruculogen + succinate + CO2 + H2O + A
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A
723320
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
-
-
-
?
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
fumitremorgin B i.e. (5aR,6S,12S,14aS)-5a,6-dihydroxy-9-methoxy-11-(3-methylbut-2-en-1-yl)-12-(2-methylprop-1-en-1-yl)-1,2,3,5a,6,11,12,14a-octahydro-5H,14H-pyrrolo[1'',2'':4',5']pyrazino[1',2':1,6]pyrido[3,4-b]indole-5,14-dione. Both atoms of a dioxygen molecule are incorporated into verruculogen
723320
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
verruculogen i.e. (5R,10S,10aR,14aS,15bS)-10,10a-dihydroxy-6-methoxy-2,2-dimethyl-5-(2-methylprop-1-en-1-yl)-1,10,10a,14,14a,15b-hexahydro-12H-3,4-dioxa-5a,11a,15a-triazacycloocta[1,2,3-lm]indeno[5,6-b]fluorene-11,15(2H,13H)-dione
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 35100, calculated from sequence; 2 * 38000, SDS-PAGE
Aspergillus fumigatus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Aspergillus fumigatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM
Aspergillus fumigatus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Aspergillus fumigatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35100
-
2 * 35100, calculated from sequence
Aspergillus fumigatus
38000
-
2 * 38000, SDS-PAGE
Aspergillus fumigatus
81000
-
gel filtration
Aspergillus fumigatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
Aspergillus fumigatus
the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A
verruculogen + succinate + CO2 + H2O + A
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
the enzyme is involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A
723320
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
-
-
-
?
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
fumitremorgin B i.e. (5aR,6S,12S,14aS)-5a,6-dihydroxy-9-methoxy-11-(3-methylbut-2-en-1-yl)-12-(2-methylprop-1-en-1-yl)-1,2,3,5a,6,11,12,14a-octahydro-5H,14H-pyrrolo[1'',2'':4',5']pyrazino[1',2':1,6]pyrido[3,4-b]indole-5,14-dione. Both atoms of a dioxygen molecule are incorporated into verruculogen
723320
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
verruculogen i.e. (5R,10S,10aR,14aS,15bS)-10,10a-dihydroxy-6-methoxy-2,2-dimethyl-5-(2-methylprop-1-en-1-yl)-1,10,10a,14,14a,15b-hexahydro-12H-3,4-dioxa-5a,11a,15a-triazacycloocta[1,2,3-lm]indeno[5,6-b]fluorene-11,15(2H,13H)-dione
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 35100, calculated from sequence; 2 * 38000, SDS-PAGE
Aspergillus fumigatus
Other publictions for EC 1.14.11.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722046
Kato
Gene disruption and biochemica ...
Aspergillus fumigatus
ChemBioChem
12
711-714
2011
1
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1
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2
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1
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2
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1
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1
1
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1
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2
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1
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1
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1
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1
1
1
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723320
Steffan
FtmOx1, a non-heme Fe(II) and ...
Aspergillus fumigatus
Org. Biomol. Chem.
7
4082-4087
2009
1
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1
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1
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1
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1
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1
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1
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1
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