BRENDA - Enzyme Database show
show all sequences of 1.14.11.38

Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus

Kato, N.; Suzuki, H.; Takagi, H.; Uramoto, M.; Takahashi, S.; Osada, H.; ChemBioChem 12, 711-714 (2011)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
not essential but involved in the FtmF reaction
Aspergillus fumigatus
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a His6-tagged fusion protein
Aspergillus fumigatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
2-oxoglutarate
pH 6.0, 30C
Aspergillus fumigatus
0.021
-
fumitremorgin B
pH 6.0, 30C
Aspergillus fumigatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77000
-
gel filtration
Aspergillus fumigatus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
-
722046
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
-
-
-
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
10 min, 10% loss of activity
Aspergillus fumigatus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.19
-
fumitremorgin B
pH 6.0, 30C
Aspergillus fumigatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Aspergillus fumigatus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
not essential but involved in the FtmF reaction
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a His6-tagged fusion protein
Aspergillus fumigatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
2-oxoglutarate
pH 6.0, 30C
Aspergillus fumigatus
0.021
-
fumitremorgin B
pH 6.0, 30C
Aspergillus fumigatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77000
-
gel filtration
Aspergillus fumigatus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
fumitremorgin B + 2-oxoglutarate + 2 O2 + AH2
-
722046
Aspergillus fumigatus
verruculogen + succinate + CO2 + H2O + A
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
10 min, 10% loss of activity
Aspergillus fumigatus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.19
-
fumitremorgin B
pH 6.0, 30C
Aspergillus fumigatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Aspergillus fumigatus
Other publictions for EC 1.14.11.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722046
Kato
Gene disruption and biochemica ...
Aspergillus fumigatus
ChemBioChem
12
711-714
2011
1
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723320
Steffan
FtmOx1, a non-heme Fe(II) and ...
Aspergillus fumigatus
Org. Biomol. Chem.
7
4082-4087
2009
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1
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