Protein Variants | Comment | Organism |
---|---|---|
D135I | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
D135N | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
E136L | the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
DNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-base-CH3 + 2-oxoglutarate + O2 | Escherichia coli | - |
DNA-base + formaldehyde + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05050 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-base-CH3 + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-base + formaldehyde + succinate + CO2 | - |
? | |
DNA-N1-methyladenine + 2-oxoglutarate + O2 | highest activity | Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1235 | - |
DNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61.8 | - |
DNA-N1-methyladenine | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |