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Literature summary for 1.14.11.33 extracted from

  • Beharry, A.A.; Lacoste, S.; OConnor, T.R.; Kool, E.T.
    Fluorescence monitoring of the oxidative repair of DNA alkylation damage by ALKBH3, a prostate cancer marker (2016), J. Am. Chem. Soc., 138, 3647-3650.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.33

Application

Application Comment Organism
analysis development of a fluorogenic probe design (MAQ) that is directly responsive to ALKBH3 repair activity. MAQ uses the fluorescence-quenching properties of 1-methyladenine, removal of the alkyl group results in an more than 10fold light-up signal. The probe is specific for ALKBH3 over its related homologue ALKBH2 and can be used to identify and measure the effectiveness of enzyme inhibitors. The probe functions efficiently in cells, allowing imaging and quantitation of ALKBH3 activity by microscopy and flow cytometry Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 1-methyl-adenine in 5'-dAAAA-1MeA-YYAAA pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
prostate
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-adenine in 5'-dAAAA-1MeA-YYAAA + 2-oxoglutarate + O2
-
 Homo sapiens 5'-dAAAAAYYAAA + formaldehyde + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ALKBH3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 1-methyl-adenine in 5'-dAAAA-1MeA-YYAAA pH not specified in the publication, temperature not specified in the publication Homo sapiens