Cloned (Comment) | Organism |
---|---|
isoforms ABH2 and ABH3 are expressed in Sf9 insect cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
poly(dm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.002 | - |
1-methyl-dAMP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.0028 | - |
d(Tpm1ApT) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.0044 | - |
d(Tpm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.0055 | - |
1-methyl-dATP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.01 | - |
N1-methyl-ATP | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.012 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | apparent value, at 37°C, pH not specified in the publication | Escherichia coli | |
0.04 | 0.05 | d(Tpm1A) | apparent value, at 37°C, pH not specified in the publication | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Escherichia coli | |
Fe2+ | required for activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Escherichia coli |
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyl-dAMP + 2-oxoglutarate + O2 | - |
Escherichia coli | dAMP + formaldehyde + succinate + CO2 | - |
? | |
1-methyl-dAMP + 2-oxoglutarate + O2 | low activity | Homo sapiens | dAMP + formaldehyde + succinate + CO2 | - |
? | |
1-methyl-dATP + 2-oxoglutarate + O2 | - |
Escherichia coli | dATP + formaldehyde + succinate + CO2 | - |
? | |
1-methyl-dATP + 2-oxoglutarate + O2 | low activity | Homo sapiens | dATP + formaldehyde + succinate + CO2 | - |
? | |
2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2 | - |
Escherichia coli | 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2 | - |
? | |
2'-deoxy-1-methyl-adenosine 3'-phosphate + 2-oxoglutarate + O2 | low activity | Homo sapiens | 2'-deoxyadenosine 3'-phosphate + formaldehyde + succinate + CO2 | - |
? | |
d(Tpm1A) + 2-oxoglutarate + O2 | - |
Escherichia coli | d(TpA) + formaldehyde + succinate + CO2 | - |
? | |
d(Tpm1A) + 2-oxoglutarate + O2 | low activity | Homo sapiens | d(TpA) + formaldehyde + succinate + CO2 | - |
? | |
d(Tpm1ApT) + 2-oxoglutarate + O2 | - |
Escherichia coli | d(TpApT) + formaldehyde + succinate + CO2 | - |
? | |
d(Tpm1ApT) + 2-oxoglutarate + O2 | low activity | Homo sapiens | d(TpApT) + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-1-methyladenine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-adenine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Escherichia coli | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
DNA-3-methylcytosine + 2-oxoglutarate + O2 | - |
Homo sapiens | DNA-cytosine + formaldehyde + succinate + CO2 | - |
? | |
N1-methyl-ATP + 2-oxoglutarate + O2 | - |
Escherichia coli | ATP + formaldehyde + succinate + CO2 | - |
? | |
N1-methyl-ATP + 2-oxoglutarate + O2 | low activity | Homo sapiens | ATP + formaldehyde + succinate + CO2 | - |
? | |
poly(dm1A) + 2-oxoglutarate + O2 | - |
Escherichia coli | poly(dA) + formaldehyde + succinate + CO2 | - |
? | |
poly(dm1A) + 2-oxoglutarate + O2 | low activity | Homo sapiens | poly(dA) + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-methyladenine-DNA dioxygenase | - |
Escherichia coli |
1-methyladenine-DNA dioxygenase | - |
Homo sapiens |
ABH2 | isoform | Homo sapiens |
ABH3 | isoform | Homo sapiens |
AlkB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | at 37°C, pH not specified in the publication | Escherichia coli | |
0.02 | - |
N1-methyl-ATP | at 37°C, pH not specified in the publication | Escherichia coli | |
0.033 | - |
1-methyl-dATP | at 37°C, pH not specified in the publication | Escherichia coli | |
0.06 | - |
d(Tpm1A) | at 37°C, pH not specified in the publication | Escherichia coli | |
0.07 | - |
1-methyl-dAMP | at 37°C, pH not specified in the publication | Escherichia coli | |
0.12 | - |
d(Tpm1ApT) | at 37°C, pH not specified in the publication | Escherichia coli | |
0.2 | - |
poly(dm1A) | at 37°C, pH not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Escherichia coli | |
2-oxoglutarate | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
1-methyl-ATP | at 37°C, pH not specified in the publication | Escherichia coli | |
3.3 | - |
2'-deoxy-1-methyl-adenosine 3'-phosphate | at 37°C, pH not specified in the publication | Escherichia coli | |
11.7 | - |
1-methyl-dATP | at 37°C, pH not specified in the publication | Escherichia coli | |
35 | - |
1-methyl-dAMP | at 37°C, pH not specified in the publication | Escherichia coli | |
43 | - |
d(Tpm1ApT) | at 37°C, pH not specified in the publication | Escherichia coli | |
143 | - |
poly(dm1A) | at 37°C, pH not specified in the publication | Escherichia coli |