Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.3 extracted from

  • Bankel, L.; Lindstedt, G.; Lindstedt, S.
    Thymidine 2-hydroxylation in Neurospora crassa (1972), J. Biol. Chem., 247, 6128-6134.
    View publication on PubMed
Search for more literature for 1.14.11.3

Activating Compound

Activating Compound Comment Organism Structure
ascorbate 3fold activation at 5 mM Neurospora crassa
catalase 1.5fold activation at 2 mg per ml Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline at 0.02 mM complete inhibition Neurospora crassa
2-ketoadipate 38% inhibition at 2.5 mM Neurospora crassa
2-ketopimelate 26% inhibition at 2.5 mM Neurospora crassa
3-ketoadipate 50% inhibition at 2.5 mM Neurospora crassa
3-oxoglutarate 27% inhibition at 2.5 mM Neurospora crassa
Co2+ 95% inhibition at 0.25 mM Neurospora crassa
Cu2+ 95% inhibition at 0.25 mM Neurospora crassa
Diglycolate 18% inhibition at 2.5 mM Neurospora crassa
glutamate 20% inhibition at 2.5 mM Neurospora crassa
iminodiacetate 28% inhibition at 2.5 mM Neurospora crassa
Mn2+ 60% inhibition at 0.25 mM Neurospora crassa
Ni2+ 95% inhibition at 0.25 mM Neurospora crassa
Thiodiglycolate 24% inhibition at 2.5 mM Neurospora crassa
Zn2+ 95% inhibition at 0.25 mM Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 thymidylate
-
 Neurospora crassa
0.09
-
 thymidine
-
 Neurospora crassa
0.15
-
 5-bromodeoxyuridine
-
 Neurospora crassa
0.19
-
 2'-deoxyuridine
-
 Neurospora crassa
0.29
-
 6-Azathymidine
-
 Neurospora crassa
0.29
-
 5-Hydroxymethyldeoxyuridine
-
 Neurospora crassa
0.3
-
 2-oxoglutarate
-
 Neurospora crassa
0.45
-
 Fe2+ at pH 7.2 in 50 mM phosphate buffer Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Neurospora crassa STA 4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyuridine + 2-oxoglutarate + O2
-
 Neurospora crassa uridine + succinate + CO2
-
 r
5-bromodeoxyuridine + 2-oxoglutarate + O2
-
 Neurospora crassa 5-bromouridine + succinate + CO2
-
 ?
5-bromodeoxyuridine + 2-oxoglutarate + O2
-
 Neurospora crassa STA 4 5-bromouridine + succinate + CO2
-
 ?
5-hydroxymethyldeoxyuridine + 2-oxoglutarate + O2
-
 Neurospora crassa 5-hydroxymethyluridine + succinate + CO2
-
 ?
5-hydroxymethyldeoxyuridine + 2-oxoglutarate + O2
-
 Neurospora crassa STA 4 5-hydroxymethyluridine + succinate + CO2
-
 ?
6-azathymidine + 2-oxoglutarate + O2
-
 Neurospora crassa 5-mehyluridine + succinate + CO2
-
 ?
6-azathymidine + 2-oxoglutarate + O2
-
 Neurospora crassa STA 4 5-mehyluridine + succinate + CO2
-
 ?
additional information only pyrimidine deoxyribonucleosides with oxygen at carbon 2 and 4 are active Neurospora crassa ?
-
 ?
additional information only pyrimidine deoxyribonucleosides with oxygen at carbon 2 and 4 are active Neurospora crassa STA 4 ?
-
 ?
thymidine + 2-oxoglutarate + O2
-
 Neurospora crassa 5-methyluridine + succinate + CO2
-
 ?
thymidylate + 2-oxoglutarate + O2
-
 Neurospora crassa 5-methyluridine 5'-phosphate + succinate + CO2
-
 ?
thymidylate + 2-oxoglutarate + O2
-
 Neurospora crassa STA 4 5-methyluridine 5'-phosphate + succinate + CO2
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Neurospora crassa