BRENDA - Enzyme Database show
show all sequences of 1.14.11.28

Substrate selectivities of proline hydroxylases

Shibasaki, T.; Sakurai, W.; Hasegawa, A.; Uosaki, Y.; Mori, H.; Yoshida, M.; Ozaki, A.; Tetrahedron Lett. 40, 5227-5230 (1999)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Streptomyces sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-proline
-
Streptomyces sp.
0.4
-
L-proline
-
Streptomyces sp.
2.1
-
L-2-azetidinecarboxylic acid
-
Streptomyces sp.
3.8
-
L-Pipecolic acid
-
Streptomyces sp.
8.4
-
3,4-dehydro-L-proline
-
Streptomyces sp.
100
-
L-Pipecolic acid
-
Streptomyces sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
-
strainTH1
-
Streptomyces sp. TH1
-
strainTH1
-
Purification (Commentary)
Commentary
Organism
2 chromatographic steps to homogeneity
Streptomyces sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3,4-epoxy-L-proline + succinate + CO2
-
-
-
?
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3,4-epoxy-L-proline + succinate + CO2
-
-
-
?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
-
-
?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
-
-
?
L-pipecolic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
-
-
?
L-pipecolic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
L-proline
-
Streptomyces sp.
0.4
-
L-proline
-
Streptomyces sp.
2.1
-
L-2-azetidinecarboxylic acid
-
Streptomyces sp.
3.8
-
L-Pipecolic acid
-
Streptomyces sp.
8.4
-
3,4-dehydro-L-proline
-
Streptomyces sp.
100
-
L-Pipecolic acid
-
Streptomyces sp.
Purification (Commentary) (protein specific)
Commentary
Organism
2 chromatographic steps to homogeneity
Streptomyces sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3,4-epoxy-L-proline + succinate + CO2
-
-
-
?
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3,4-epoxy-L-proline + succinate + CO2
-
-
-
?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
-
-
?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
-
-
?
L-pipecolic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
-
-
?
L-pipecolic acid + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp.
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
L-proline + 2-oxoglutarate + O2
-
666959
Streptomyces sp. TH1
cis-3-hydroxy-L-proline + succinate + CO2
-
-
-
?
Other publictions for EC 1.14.11.28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Hatzimichael
The collagen prolyl hydroxylas ...
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1423-1432
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1
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1
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1
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1
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726654
Mordechai
High myopia caused by a mutati ...
Homo sapiens
Am. J. Hum. Genet.
89
438-445
2011
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-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
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1
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
697017
Shah
The prolyl 3-hydroxylases P3H2 ...
Homo sapiens
Br. J. Cancer
100
1687-1696
2009
-
1
-
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-
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2
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2
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-
-
-
-
-
-
-
-
-
-
1
1
2
2
-
-
697667
Johnston
-
Hydroxylation of L-proline to ...
Streptomyces sp., Streptomyces sp. TH1
Enzyme Microb. Technol.
45
484-490
2009
-
-
1
-
-
-
-
-
-
1
1
2
-
7
-
-
-
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
665517
Vranka
Prolyl 3-hydroxylase 1, enzyme ...
Gallus gallus
J. Biol. Chem.
279
23615-23621
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664814
Clifton
Structure of proline 3-hydroxy ...
Streptomyces sp., Streptomyces sp. TH1
Eur. J. Biochem.
268
6625-6636
2001
-
-
-
1
-
-
-
-
-
-
-
2
-
8
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664403
Shibasaki
-
Cloning of an isozyme of proli ...
Streptomyces sp., Streptomyces sp. TH1
Biotechnol. Lett.
22
1967-1973
2000
-
-
1
-
-
-
5
4
-
1
3
-
-
7
-
-
1
-
-
1
2
-
8
-
2
-
2
-
1
-
2
1
-
2
-
-
-
1
1
-
-
-
-
5
-
4
-
1
3
-
-
-
-
1
-
1
2
-
8
-
2
-
2
-
1
-
2
2
-
-
-
-
-
-
666959
Shibasaki
-
Substrate selectivities of pro ...
Streptomyces sp., Streptomyces sp. TH1
Tetrahedron Lett.
40
5227-5230
1999
-
-
1
-
-
-
-
6
-
-
-
-
-
7
-
-
1
-
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665361
Mori
Purification and cloning of a ...
Streptomyces sp., Streptomyces sp. TH1
J. Bacteriol.
179
5677-5683
1997
1
-
-
-
-
-
5
2
-
1
1
-
-
10
-
-
1
-
-
1
-
-
2
-
1
-
-
1
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
5
-
2
-
1
1
-
-
-
-
1
-
1
-
-
2
-
1
-
-
1
1
-
-
1
-
-
-
-
-
-
663681
Mori
Detection of novel proline 3-h ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) TH2, Bacillus sp. (in: Bacteria) TH3, Streptomyces canus, Streptomyces sp., Streptomyces sp. TH1
Appl. Environ. Microbiol.
62
1903-1907
1996
-
-
-
-
-
-
4
-
-
3
-
-
-
16
-
-
-
-
-
3
4
-
6
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
-
-
3
-
-
-
-
-
-
-
3
4
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-