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Literature summary for 1.14.11.28 extracted from

  • Shibasaki, T.; Mori, H.; Ozaki, A.
    Cloning of an isozyme of proline 3-hydroxylase and its purification from recombinant Escherichia coli (2000), Biotechnol. Lett., 22, 1967-1973.
No PubMed abstract available
Search for more literature for 1.14.11.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ more than 50% inhibition at 1 mM Streptomyces sp.
Cu2+ more than 50% inhibition at 1 mM Streptomyces sp.
EDTA more than 50% inhibition at 1 mM Streptomyces sp.
Ni2+ more than 50% inhibition at 1 mM Streptomyces sp.
Zn2+ more than 50% inhibition at 1 mM Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 2-oxoglutarate
-
 Streptomyces sp.
0.083
-
 2-oxoglutarate
-
 Streptomyces sp.
0.2
-
 L-proline
-
 Streptomyces sp.
0.43
-
 L-proline
-
 Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ strictly required Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33150
-
 calculated Streptomyces sp.
33570
-
 calculated Streptomyces sp.
35000
-
 apparent molecular weight on SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 strainTH1
-
Streptomyces sp. TH1
-
 strainTH1
-

Purification (Commentary)

Purification (Comment) Organism
2 chromatographic steps to homogeneity Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2
-
-
 Streptomyces sp.
12.7
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
proline 3-hydroxylase type I
-
 Streptomyces sp.
proline 3-hydroxylase type II
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Streptomyces sp.
40
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 below 30°C activity remained above 85% after 30 min at pH 6.0 Streptomyces sp.
40
-
 below 40°C activity remained above 85% after 30 min at pH 6.0 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 activity remained above 85% after 16 h at 4°C Streptomyces sp.
5.5 8.5 activity remained above 85% after 16 h at 4°C Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
2-oxoglutarate strictly required Streptomyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp.
-
-
 4.3
Streptomyces sp.
-
-
 4.6