Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 82% inhibition at 0.1 mM, 98% inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C | Streptomyces sp. | |
CuSO4 | 59% inhibition at 0.1 mM, complete inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C | Streptomyces sp. | |
EDTA | 95% inhibition at 2 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C | Streptomyces sp. | |
ZnSO4 | 95% inhibition at 0.1 mM, complete inhibition at 1 mM, 5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM added to cell extracts, 60% inhibition without addition of Fe2+ | Bacillus sp. (in: Bacteria) | |
Fe2+ | 1 mM added to cell extracts, 60% inhibition without addition of Fe2+ | Streptomyces sp. | |
Fe2+ | 1 mM added to cell extracts, 85% inhibition without addition of Fe2+ | Bacillus sp. (in: Bacteria) | |
Fe2+ | 1 mM added to cell extracts, complete inhibition without addition of Fe2+ | Streptomyces canus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) TH2 | - |
- |
- |
Bacillus sp. (in: Bacteria) TH3 | - |
- |
- |
Streptomyces canus | - |
strain ATCC 12647 | - |
Streptomyces sp. | - |
- |
- |
Streptomyces sp. TH1 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bacillus sp. (in: Bacteria) | - |
cell culture | - |
Streptomyces sp. | - |
cell culture | - |
Streptomyces canus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00029 | - |
- |
Streptomyces canus |
0.00119 | - |
5 mM 2-oxoglutarate, 1 mM ferrous sulfate, 5 mM L-ascorbic acid, 100 mM TES pH 7.5, 30°C | Streptomyces sp. |
0.00132 | - |
- |
Bacillus sp. (in: Bacteria) |
0.00149 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2-oxoglutarate + O2 | - |
Bacillus sp. (in: Bacteria) | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces canus | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Bacillus sp. (in: Bacteria) TH2 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Bacillus sp. (in: Bacteria) TH3 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? | |
L-proline + 2-oxoglutarate + O2 | - |
Streptomyces sp. TH1 | cis-3-hydroxy-L-proline + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
proline 3-hydroxylase | - |
Bacillus sp. (in: Bacteria) |
proline 3-hydroxylase | - |
Streptomyces sp. |
proline 3-hydroxylase | - |
Streptomyces canus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | strictly required, highest activity at 4 mM | Bacillus sp. (in: Bacteria) | |
2-oxoglutarate | strictly required, highest activity at 4 mM | Streptomyces sp. | |
2-oxoglutarate | strictly required, highest activity at 4 mM | Streptomyces canus |