BRENDA - Enzyme Database
show all sequences of 1.14.11.26

Expression of cefF significantly decreased deacetoxycephalosporin C formation during cephalosporin C production in Acremonium chrysogenum

An, Y.; Dong, H.; Liu, G.; J. Ind. Microbiol. Biotechnol. 39, 269-274 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene cefEF
Acremonium chrysogenum
gene cefF, subcloning in Escherichia coli, functional expression in Acremonium chrysogenum strain CGMCC3.3795 via Agrobacterium tumefaciens-mediated transformation under the promoter of pcbC, leading to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis
Streptomyces clavuligerus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Acremonium chrysogenum
Fe2+
required
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
Acremonium chrysogenum
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Acremonium chrysogenum CGMCC3.3795
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Streptomyces clavuligerus CGMCC4.1611
-
deacetylcephalosporin C + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Acremonium chrysogenum
P11935
formerly Cephalosporium acremonium, gene cefEF
-
Acremonium chrysogenum CGMCC3.3795
P11935
formerly Cephalosporium acremonium, gene cefEF
-
Streptomyces clavuligerus
P42220
gene cefF
-
Streptomyces clavuligerus CGMCC4.1611
P42220
gene cefF
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Acremonium chrysogenum
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Streptomyces clavuligerus
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Acremonium chrysogenum CGMCC3.3795
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Streptomyces clavuligerus CGMCC4.1611
deacetylcephalosporin C + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Cephalosporin biosynthesis expandase/hydroxylase
UniProt
Acremonium chrysogenum
DAOC synthase
-
Streptomyces clavuligerus
DAOC/DAC synthase
-
Acremonium chrysogenum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cefEF
Acremonium chrysogenum
gene cefF, subcloning in Escherichia coli, functional expression in Acremonium chrysogenum strain CGMCC3.3795 via Agrobacterium tumefaciens-mediated transformation under the promoter of pcbC, leading to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis
Streptomyces clavuligerus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Acremonium chrysogenum
Fe2+
required
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
Acremonium chrysogenum
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Acremonium chrysogenum CGMCC3.3795
-
deacetylcephalosporin C + succinate + CO2
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
Streptomyces clavuligerus CGMCC4.1611
-
deacetylcephalosporin C + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Acremonium chrysogenum
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Streptomyces clavuligerus
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Acremonium chrysogenum CGMCC3.3795
deacetylcephalosporin C + succinate + CO2
-
-
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
725639
Streptomyces clavuligerus CGMCC4.1611
deacetylcephalosporin C + succinate + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains
Acremonium chrysogenum
metabolism
two enzymes, deacetoxycephalosporin C synthase and deacetoxycephalosporin C hydroxylase, are involved in the conversion of penicillin N into deacetylcephalosporin C in Streptomyces clavuligerus
Streptomyces clavuligerus
additional information
additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview
Acremonium chrysogenum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains
Acremonium chrysogenum
metabolism
two enzymes, deacetoxycephalosporin C synthase and deacetoxycephalosporin C hydroxylase, are involved in the conversion of penicillin N into deacetylcephalosporin C in Streptomyces clavuligerus
Streptomyces clavuligerus
additional information
additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview
Acremonium chrysogenum
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744105
Balakrishnan
Modified deacetylcephalospori ...
Streptomyces clavuligerus, Streptomyces clavuligerus ATCC 27064
Appl. Environ. Microbiol.
82
3711-3720
2016
1
-
1
-
53
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5
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1
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3
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4
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3
1
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6
1
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1
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1
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53
-
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5
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1
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2
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4
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1
-
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6
1
-
-
-
-
4
4
-
6
6
725639
An
Expression of cefF significant ...
Acremonium chrysogenum, Acremonium chrysogenum CGMCC3.3795, Streptomyces clavuligerus, Streptomyces clavuligerus CGMCC4.1611
J. Ind. Microbiol. Biotechnol.
39
269-274
2012
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8
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2
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4
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4
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-
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3
3
-
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-
699366
Goo
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Streptomyces clavuligerus
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619-633
2009
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1
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700004
Liras
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1
-
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Wu
C-terminus mutations of Acremo ...
Acremonium chrysogenum
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Wei
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28
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33
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18
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28
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Controlling the substrate sele ...
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5
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Acremonium chrysogenum
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24
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Characterization of the cefF g ...
Amycolatopsis lactamdurans
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Streptomyces clavuligerus
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