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Literature summary for 1.14.11.26 extracted from

  • Liras, P.; Demain, A.L.
    Enzymology of beta-lactam compounds with cephem structure produced by actinomycete (2009), Methods Enzymol., 458, 401-429.
    View publication on PubMed

Application

Application Comment Organism
biotechnology production of beta-lactam antibiotics Actinomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
2-oxoglutarate
-
Actinomyces sp.
0.02
-
Fe2+
-
Actinomyces sp.
0.059
-
deacetoxycephalosporin C
-
Actinomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ essential fpr DAOC hydrolase activity, enzyme is a nonheme Fe2+ oxygenase Actinomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-exomethylenecephalosporin + 2-oxoglutarate + O2 Actinomyces sp.
-
deacetoxycephalosporin C + succinate + CO2 + H2O
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2 Actinomyces sp. hydroxylation at C-3' by deacetylcephalosporin C hydroxylase deacetylcephalosporin C + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Actinomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DAOC hydroxylase of Streptomyces clavuligerus is purified to near homogeneity by anion-exchange chromatography, ammonium sulphate precipitation and gel filtration Actinomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-exomethylenecephalosporin + 2-oxoglutarate + O2
-
Actinomyces sp. deacetoxycephalosporin C + succinate + CO2 + H2O
-
?
deacetoxycephalosporin C + 2-oxoglutarate + O2 hydroxylation at C-3' by deacetylcephalosporin C hydroxylase Actinomyces sp. deacetylcephalosporin C + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
DAOC hydroxylase
-
Actinomyces sp.
deacetoxycephalosporin C hydroxylase
-
Actinomyces sp.