Application | Comment | Organism |
---|---|---|
biotechnology | production of beta-lactam antibiotics | Actinomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2-oxoglutarate | - |
Actinomyces sp. | |
0.02 | - |
Fe2+ | - |
Actinomyces sp. | |
0.059 | - |
deacetoxycephalosporin C | - |
Actinomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | essential fpr DAOC hydrolase activity, enzyme is a nonheme Fe2+ oxygenase | Actinomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-exomethylenecephalosporin + 2-oxoglutarate + O2 | Actinomyces sp. | - |
deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
deacetoxycephalosporin C + 2-oxoglutarate + O2 | Actinomyces sp. | hydroxylation at C-3' by deacetylcephalosporin C hydroxylase | deacetylcephalosporin C + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DAOC hydroxylase of Streptomyces clavuligerus is purified to near homogeneity by anion-exchange chromatography, ammonium sulphate precipitation and gel filtration | Actinomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-exomethylenecephalosporin + 2-oxoglutarate + O2 | - |
Actinomyces sp. | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
deacetoxycephalosporin C + 2-oxoglutarate + O2 | hydroxylation at C-3' by deacetylcephalosporin C hydroxylase | Actinomyces sp. | deacetylcephalosporin C + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAOC hydroxylase | - |
Actinomyces sp. |
deacetoxycephalosporin C hydroxylase | - |
Actinomyces sp. |