Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner(DE3) and BL21(DE3) cells | Streptomyces clavuligerus |
Protein Variants | Comment | Organism |
---|---|---|
A106T | 80% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
C155Y | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
C155Y/Y184H/V275I/C281Y | 580% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
C281Y | 200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
G300V | 410% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
G79E | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
H244Q | 140% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
I305L | 230% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
I305M | 380% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
L277Q | 270% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
M188I | 90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
M188V | 150% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
M73T | 180% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
N304K | 220% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
T91A | 110% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
V275I | 270% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
V275I/I305M | 500% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
Y184H | 200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Penicillin N | mutant enzyme N304K | Streptomyces clavuligerus | |
0.006 | - |
Penicillin N | mutant enzyme C281Y | Streptomyces clavuligerus | |
0.006 | - |
Penicillin N | mutant enzyme I305L | Streptomyces clavuligerus | |
0.006 | - |
Penicillin N | mutant enzyme M73T | Streptomyces clavuligerus | |
0.009 | - |
Penicillin N | mutant enzyme G79E | Streptomyces clavuligerus | |
0.009 | - |
Penicillin N | mutant enzyme H244Q | Streptomyces clavuligerus | |
0.009 | - |
Penicillin N | mutant enzyme T91A | Streptomyces clavuligerus | |
0.011 | - |
Penicillin N | mutant enzyme L277Q | Streptomyces clavuligerus | |
0.011 | - |
Penicillin N | mutant enzyme M188I | Streptomyces clavuligerus | |
0.012 | - |
Penicillin N | mutant enzyme I305M | Streptomyces clavuligerus | |
0.012 | - |
Penicillin N | mutant enzyme V275I | Streptomyces clavuligerus | |
0.013 | - |
Penicillin N | mutant enzyme A106T | Streptomyces clavuligerus | |
0.013 | - |
Penicillin N | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
0.014 | - |
Penicillin N | wild type enzyme | Streptomyces clavuligerus | |
0.016 | - |
Penicillin N | mutant enzyme C155Y | Streptomyces clavuligerus | |
0.047 | - |
Penicillin N | mutant enzyme M188V | Streptomyces clavuligerus | |
0.092 | - |
Penicillin N | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
0.19 | - |
penicillin G | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
0.22 | - |
penicillin G | mutant enzyme N304K | Streptomyces clavuligerus | |
0.25 | - |
penicillin G | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
0.295 | - |
Penicillin N | mutant enzyme Y184H | Streptomyces clavuligerus | |
0.66 | - |
penicillin G | mutant enzyme I305L | Streptomyces clavuligerus | |
0.68 | - |
penicillin G | mutant enzyme C281Y | Streptomyces clavuligerus | |
0.74 | - |
penicillin G | mutant enzyme M73T | Streptomyces clavuligerus | |
0.75 | - |
penicillin G | mutant enzyme G79E | Streptomyces clavuligerus | |
0.75 | - |
penicillin G | mutant enzyme I305M | Streptomyces clavuligerus | |
0.76 | - |
penicillin G | mutant enzyme M188V | Streptomyces clavuligerus | |
0.95 | - |
penicillin G | mutant enzyme Y184H | Streptomyces clavuligerus | |
1.02 | - |
penicillin G | mutant enzyme L277Q | Streptomyces clavuligerus | |
1.36 | - |
penicillin G | mutant enzyme T91A | Streptomyces clavuligerus | |
1.39 | - |
penicillin G | mutant enzyme H244Q | Streptomyces clavuligerus | |
1.68 | - |
penicillin G | mutant enzyme V275I | Streptomyces clavuligerus | |
1.76 | - |
penicillin G | mutant enzyme C155Y | Streptomyces clavuligerus | |
1.77 | - |
penicillin G | mutant enzyme A106T | Streptomyces clavuligerus | |
1.96 | - |
penicillin G | mutant enzyme M188I | Streptomyces clavuligerus | |
2.58 | - |
penicillin G | wild type enzyme | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin N + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
? + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion-exchange chromatography and gel filtration | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 | - |
? | |
penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
phenylacetyl-7-aminodeacetoxycephalosporanic acid + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetylcephalosporin + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAOCS | - |
Streptomyces clavuligerus |
deacetoxycephalosporin C synthase | - |
Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0315 | - |
penicillin G | mutant enzyme G79E | Streptomyces clavuligerus | |
0.0453 | - |
penicillin G | wild type enzyme | Streptomyces clavuligerus | |
0.0453 | - |
penicillin G | mutant enzyme A106T | Streptomyces clavuligerus | |
0.0456 | - |
penicillin G | mutant enzyme M188V | Streptomyces clavuligerus | |
0.0476 | - |
penicillin G | mutant enzyme C155Y | Streptomyces clavuligerus | |
0.048 | - |
Penicillin N | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
0.048 | - |
Penicillin N | mutant enzyme Y184H | Streptomyces clavuligerus | |
0.0502 | - |
penicillin G | mutant enzyme V275I | Streptomyces clavuligerus | |
0.0507 | - |
penicillin G | mutant enzyme M188I | Streptomyces clavuligerus | |
0.0522 | - |
penicillin G | mutant enzyme T91A | Streptomyces clavuligerus | |
0.0564 | - |
penicillin G | mutant enzyme N304K | Streptomyces clavuligerus | |
0.0627 | - |
penicillin G | mutant enzyme M73T | Streptomyces clavuligerus | |
0.0698 | - |
penicillin G | mutant enzyme H244Q | Streptomyces clavuligerus | |
0.0744 | - |
penicillin G | mutant enzyme C281Y | Streptomyces clavuligerus | |
0.0759 | - |
penicillin G | mutant enzyme I305L | Streptomyces clavuligerus | |
0.0798 | - |
penicillin G | mutant enzyme Y184H | Streptomyces clavuligerus | |
0.084 | - |
Penicillin N | mutant enzyme M188V | Streptomyces clavuligerus | |
0.1036 | - |
penicillin G | mutant enzyme L277Q | Streptomyces clavuligerus | |
0.1398 | - |
penicillin G | mutant enzyme C155Y/Y184/V275I/C281Y | Streptomyces clavuligerus | |
0.1452 | - |
penicillin G | mutant enzyme I305M | Streptomyces clavuligerus | |
0.1458 | - |
penicillin G | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
0.178 | - |
Penicillin N | mutant enzyme G79E | Streptomyces clavuligerus | |
0.235 | - |
Penicillin N | mutant enzyme C155Y | Streptomyces clavuligerus | |
0.239 | - |
Penicillin N | mutant enzyme M73T | Streptomyces clavuligerus | |
0.247 | - |
Penicillin N | mutant enzyme T91A | Streptomyces clavuligerus | |
0.252 | - |
Penicillin N | mutant enzyme V275I | Streptomyces clavuligerus | |
0.26 | - |
Penicillin N | mutant enzyme H244Q | Streptomyces clavuligerus | |
0.273 | - |
Penicillin N | mutant enzyme C281Y | Streptomyces clavuligerus | |
0.277 | - |
Penicillin N | mutant enzyme A106T | Streptomyces clavuligerus | |
0.277 | - |
Penicillin N | mutant enzyme M188I | Streptomyces clavuligerus | |
0.284 | - |
Penicillin N | mutant enzyme I305L | Streptomyces clavuligerus | |
0.297 | - |
Penicillin N | mutant enzyme L277Q | Streptomyces clavuligerus | |
0.307 | - |
Penicillin N | wild type enzyme | Streptomyces clavuligerus | |
0.31 | - |
Penicillin N | mutant enzyme I305M | Streptomyces clavuligerus | |
0.316 | - |
Penicillin N | mutant enzyme V275I/I305M | Streptomyces clavuligerus | |
0.366 | - |
Penicillin N | mutant enzyme N304K | Streptomyces clavuligerus |