Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Streptomyces clavuligerus | |
glutathione | reduced | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, no residual activity | Streptomyces clavuligerus | |
EDTA | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
iodoacetic acid | 1 mM, 19% residual activity | Streptomyces clavuligerus | |
N-ethylmaleimide | 1 mM, no residual activity | Streptomyces clavuligerus | |
o-phenanthroline | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
p-hydroxymercuribenzoate | 0.1 mM, no residual activity | Streptomyces clavuligerus | |
Penicillin N | 1 mM, 62% inhibition, competitive with deacetoxycephalosporin C | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, without Fe2+, less than 2% of maximum activity | Streptomyces clavuligerus | |
additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
also has slight activity of EC 1.14.20.1, ring expansion reaction of penicillin N | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-exo-methylenecephalosporin C + 2-oxoglutarate + O2 | 37% of activity with deacetoxycephalosporin C | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 | - |
? | |
deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
Penicillin N | pH 7.3, 29°C | Streptomyces clavuligerus |