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Literature summary for 1.14.11.26 extracted from
- Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication (1991), J. Biol. Chem., 266, 5087-5093.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Streptomyces clavuligerus |  |
| glutathione | reduced | Streptomyces clavuligerus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, no residual activity | Streptomyces clavuligerus | |
| EDTA | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
| iodoacetic acid | 1 mM, 19% residual activity | Streptomyces clavuligerus | |
| N-ethylmaleimide | 1 mM, no residual activity | Streptomyces clavuligerus | |
| o-phenanthroline | 0.5 mM, no residual activity | Streptomyces clavuligerus | |
| p-hydroxymercuribenzoate | 0.1 mM, no residual activity | Streptomyces clavuligerus | |
| Penicillin N | 1 mM, 62% inhibition, competitive with deacetoxycephalosporin C | Streptomyces clavuligerus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required, without Fe2+, less than 2% of maximum activity | Streptomyces clavuligerus | |
| additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Streptomyces clavuligerus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
also has slight activity of EC 1.14.20.1, ring expansion reaction of penicillin N | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-exo-methylenecephalosporin C + 2-oxoglutarate + O2 | 37% of activity with deacetoxycephalosporin C | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 | - |
? | |
| deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
Penicillin N | pH 7.3, 29°C | Streptomyces clavuligerus | |
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