BRENDA - Enzyme Database
show all sequences of 1.14.11.26

Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium

Dotzlaf, J.E.; Yeh, W.K.; J. Bacteriol. 169, 1611-1618 (1987)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoic acid
1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
Ca2+
weak
Acremonium chrysogenum
EDTA
0.6 mM, 83% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
iodoacetic acid
1 mM, 2% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
N-ethylmaleimide
1 mM, 97% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
o-phenanthroline
0.6 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
p-hydroxymercuribenzoate
1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
Zn2+
strong
Acremonium chrysogenum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
deacetoxycephalosporin C
pH 7.5, 36C
Acremonium chrysogenum
0.022
-
2-oxoglutarate
pH 7.5, 36C
Acremonium chrysogenum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for both activities
Acremonium chrysogenum
additional information
Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+
Acremonium chrysogenum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
1 * 41000, SDS-PAGE
Acremonium chrysogenum
43000
-
gel filtration
Acremonium chrysogenum
Organism
Organism
UniProt
Commentary
Textmining
Acremonium chrysogenum
-
catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.127
-
pH 7.5, 36C
Acremonium chrysogenum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
661974
Acremonium chrysogenum
deacetylcephalosporin C + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Acremonium chrysogenum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
36
38
-
Acremonium chrysogenum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Acremonium chrysogenum
isoelectric focusing
-
6.3
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoic acid
1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
Ca2+
weak
Acremonium chrysogenum
EDTA
0.6 mM, 83% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
iodoacetic acid
1 mM, 2% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
N-ethylmaleimide
1 mM, 97% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
o-phenanthroline
0.6 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
p-hydroxymercuribenzoate
1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
Zn2+
strong
Acremonium chrysogenum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
deacetoxycephalosporin C
pH 7.5, 36C
Acremonium chrysogenum
0.022
-
2-oxoglutarate
pH 7.5, 36C
Acremonium chrysogenum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for both activities
Acremonium chrysogenum
additional information
Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+
Acremonium chrysogenum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
1 * 41000, SDS-PAGE
Acremonium chrysogenum
43000
-
gel filtration
Acremonium chrysogenum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.127
-
pH 7.5, 36C
Acremonium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
deacetoxycephalosporin C + 2-oxoglutarate + O2
-
661974
Acremonium chrysogenum
deacetylcephalosporin C + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 41000, SDS-PAGE
Acremonium chrysogenum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
36
38
-
Acremonium chrysogenum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
hydroxylation reaction, EC 1.14.11.26
Acremonium chrysogenum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Acremonium chrysogenum
isoelectric focusing
-
6.3
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744105
Balakrishnan
Modified deacetylcephalospori ...
Streptomyces clavuligerus, Streptomyces clavuligerus ATCC 27064
Appl. Environ. Microbiol.
82
3711-3720
2016
1
-
1
-
53
-
-
5
-
1
-
2
-
3
-
-
-
-
-
-
-
-
4
-
3
1
-
-
6
1
-
-
-
-
-
-
1
-
1
-
-
53
-
-
-
-
5
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
6
1
-
-
-
-
4
4
-
6
6
725639
An
Expression of cefF significant ...
Acremonium chrysogenum, Acremonium chrysogenum CGMCC3.3795, Streptomyces clavuligerus, Streptomyces clavuligerus CGMCC4.1611
J. Ind. Microbiol. Biotechnol.
39
269-274
2012
-
-
2
-
-
-
-
-
-
2
-
4
-
8
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
699366
Goo
Directed evolution and rationa ...
Streptomyces clavuligerus
J. Ind. Microbiol. Biotechnol.
36
619-633
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700004
Liras
Enzymology of beta-lactam comp ...
Actinomyces sp.
Methods Enzymol.
458
401-429
2009
-
1
-
-
-
-
-
3
-
1
-
2
-
1
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671324
Stok
Development of enzyme-linked i ...
Streptomyces clavuligerus
Anal. Chim. Acta
577
153-162
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661796
Wu
C-terminus mutations of Acremo ...
Acremonium chrysogenum
FEMS Microbiol. Lett.
246
103-110
2005
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671443
Wei
Directed evolution of Streptom ...
Streptomyces clavuligerus
Appl. Environ. Microbiol.
71
8873-8880
2005
-
-
1
-
18
-
-
28
-
1
-
1
-
1
-
-
1
-
-
-
-
-
4
-
2
-
-
-
33
-
-
-
-
-
-
-
-
-
1
-
-
18
-
-
-
-
28
-
1
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
33
-
-
-
-
-
-
-
-
-
-
659322
Lloyd
Controlling the substrate sele ...
Acremonium chrysogenum
J. Biol. Chem.
279
15420-15426
2004
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440375
Ghag
Refolding and purification of ...
Acremonium chrysogenum
Biotechnol. Appl. Biochem.
24
109-119
1996
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660772
Coque
Characterization of the cefF g ...
Amycolatopsis lactamdurans
Appl. Microbiol. Biotechnol.
44
605-609
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
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-
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1
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-
-
-
-
-
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1
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-
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1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662088
Baker
Deacetoxycephalosporin C hydro ...
Streptomyces clavuligerus
J. Biol. Chem.
266
5087-5093
1991
2
-
-
-
-
-
7
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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1
-
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2
-
-
-
-
-
-
-
7
1
-
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2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
440380
Dotzlaf
Purification and properties of ...
Streptomyces clavuligerus
J. Biol. Chem.
264
10219-10227
1989
2
-
1
-
-
-
12
-
-
1
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
1
1
-
1
-
1
-
-
2
-
2
-
1
-
-
-
-
-
12
-
-
-
1
1
-
-
-
-
1
1
-
-
-
1
-
-
1
1
-
1
-
1
2
-
-
-
-
-
-
660843
Samson
-
Cloning and expression of the ...
Acremonium chrysogenum
Bio/Technology
5
1207-1214
1987
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
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1
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-
-
-
-
-
-
-
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1
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2
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
660936
Baldwin
Purification and initial chara ...
Acremonium chrysogenum
Biochem. J.
245
831-841
1987
4
-
-
-
-
1
4
-
-
-
1
-
-
2
-
-
1
-
-
-
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2
1
1
-
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-
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1
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-
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4
-
-
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-
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1
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4
-
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1
-
-
-
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1
-
-
-
2
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
661974
Dotzlaf
Copurification and characteriz ...
Acremonium chrysogenum
J. Bacteriol.
169
1611-1618
1987
-
-
-
-
-
-
8
2
-
2
2
-
-
2
-
-
-
-
-
-
1
-
1
1
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1
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-
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1
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1
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8
-
2
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2
2
-
-
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-
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-
-
1
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
661955
Jensen
Deacetoxycephalosporin C synth ...
Streptomyces clavuligerus
J. Antibiot.
38
263-265
1985
-
-
-
-
-
-
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1
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1
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2
-
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1
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1
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1
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1
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