Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.26 extracted from

  • Dotzlaf, J.E.; Yeh, W.K.
    Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus (1989), J. Biol. Chem., 264, 10219-10227.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate
-
Streptomyces clavuligerus
dithiothreitol
-
Streptomyces clavuligerus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces clavuligerus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoic acid reactivation by dithiothreitol Streptomyces clavuligerus
ampicillin
-
Streptomyces clavuligerus
Co2+ inhibition in decreasing order, Zn2+, Co2+, Ni2+ Streptomyces clavuligerus
EDTA
-
Streptomyces clavuligerus
iodoacetic acid
-
Streptomyces clavuligerus
N-ethylmaleimide
-
Streptomyces clavuligerus
Ni2+ inhibition in decreasing order, Zn2+, Co2+, Ni2+ Streptomyces clavuligerus
o-phenanthroline
-
Streptomyces clavuligerus
p-hydroxymercuribenzoate
-
Streptomyces clavuligerus
penicillin G
-
Streptomyces clavuligerus
Penicillin V
-
Streptomyces clavuligerus
Zn2+ inhibition in decreasing order, Zn2+, Co2+, Ni2+ Streptomyces clavuligerus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, Ka-value 0.008 mM Streptomyces clavuligerus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus
-
native enzyme and recombinant enzyme expressed in Escherichia coli, enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, partial, recombinant enzyme, to near homogenity Streptomyces clavuligerus

Renatured (Commentary)

Renatured (Comment) Organism
enzyme inactivated by 5,5’-dithiobis-2-nitrobenzoic acid, complete reactivation by dithiothreitol Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-exomethylenecephalosporin C + 2-oxoglutarate + O2
-
Streptomyces clavuligerus deacetylcephalosporin C + succinate + CO2 + H2O
-
?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
36
-
-
Streptomyces clavuligerus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Streptomyces clavuligerus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Streptomyces clavuligerus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9
-
Streptomyces clavuligerus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces clavuligerus isoelectric focusing
-
5.3
Streptomyces clavuligerus isoelectric focusing
-
6.1