Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Streptomyces clavuligerus | |
dithiothreitol | - |
Streptomyces clavuligerus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoic acid | reactivation by dithiothreitol | Streptomyces clavuligerus | |
ampicillin | - |
Streptomyces clavuligerus | |
Co2+ | inhibition in decreasing order, Zn2+, Co2+, Ni2+ | Streptomyces clavuligerus | |
EDTA | - |
Streptomyces clavuligerus | |
iodoacetic acid | - |
Streptomyces clavuligerus | |
N-ethylmaleimide | - |
Streptomyces clavuligerus | |
Ni2+ | inhibition in decreasing order, Zn2+, Co2+, Ni2+ | Streptomyces clavuligerus | |
o-phenanthroline | - |
Streptomyces clavuligerus | |
p-hydroxymercuribenzoate | - |
Streptomyces clavuligerus | |
penicillin G | - |
Streptomyces clavuligerus | |
Penicillin V | - |
Streptomyces clavuligerus | |
Zn2+ | inhibition in decreasing order, Zn2+, Co2+, Ni2+ | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, Ka-value 0.008 mM | Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
native enzyme and recombinant enzyme expressed in Escherichia coli, enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
Purification (Comment) | Organism |
---|---|
native enzyme, partial, recombinant enzyme, to near homogenity | Streptomyces clavuligerus |
Renatured (Comment) | Organism |
---|---|
enzyme inactivated by 5,5-dithiobis-2-nitrobenzoic acid, complete reactivation by dithiothreitol | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-exomethylenecephalosporin C + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetylcephalosporin C + succinate + CO2 + H2O | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Streptomyces clavuligerus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces clavuligerus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Streptomyces clavuligerus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces clavuligerus | isoelectric focusing | - |
5.3 |
Streptomyces clavuligerus | isoelectric focusing | - |
6.1 |