BRENDA - Enzyme Database
show all sequences of 1.14.11.26

Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus

Dotzlaf, J.E.; Yeh, W.K.; J. Biol. Chem. 264, 10219-10227 (1989)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
-
Streptomyces clavuligerus
dithiothreitol
-
Streptomyces clavuligerus
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces clavuligerus
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoic acid
reactivation by dithiothreitol
Streptomyces clavuligerus
ampicillin
-
Streptomyces clavuligerus
Co2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
EDTA
-
Streptomyces clavuligerus
iodoacetic acid
-
Streptomyces clavuligerus
N-ethylmaleimide
-
Streptomyces clavuligerus
Ni2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
o-phenanthroline
-
Streptomyces clavuligerus
p-hydroxymercuribenzoate
-
Streptomyces clavuligerus
penicillin G
-
Streptomyces clavuligerus
Penicillin V
-
Streptomyces clavuligerus
Zn2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required, Ka-value 0.008 mM
Streptomyces clavuligerus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Streptomyces clavuligerus
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces clavuligerus
-
native enzyme and recombinant enzyme expressed in Escherichia coli, enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme, partial, recombinant enzyme, to near homogenity
Streptomyces clavuligerus
Renatured (Commentary)
Renatured (Commentary)
Organism
enzyme inactivated by 5,5’-dithiobis-2-nitrobenzoic acid, complete reactivation by dithiothreitol
Streptomyces clavuligerus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-exomethylenecephalosporin C + 2-oxoglutarate + O2
-
440380
Streptomyces clavuligerus
deacetylcephalosporin C + succinate + CO2 + H2O
-
-
-
?
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
36
-
-
Streptomyces clavuligerus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable up to
Streptomyces clavuligerus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces clavuligerus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9
-
Streptomyces clavuligerus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces clavuligerus
isoelectric focusing
-
5.3
Streptomyces clavuligerus
isoelectric focusing
-
6.1
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
-
Streptomyces clavuligerus
dithiothreitol
-
Streptomyces clavuligerus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces clavuligerus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-2-nitrobenzoic acid
reactivation by dithiothreitol
Streptomyces clavuligerus
ampicillin
-
Streptomyces clavuligerus
Co2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
EDTA
-
Streptomyces clavuligerus
iodoacetic acid
-
Streptomyces clavuligerus
N-ethylmaleimide
-
Streptomyces clavuligerus
Ni2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
o-phenanthroline
-
Streptomyces clavuligerus
p-hydroxymercuribenzoate
-
Streptomyces clavuligerus
penicillin G
-
Streptomyces clavuligerus
Penicillin V
-
Streptomyces clavuligerus
Zn2+
inhibition in decreasing order, Zn2+, Co2+, Ni2+
Streptomyces clavuligerus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required, Ka-value 0.008 mM
Streptomyces clavuligerus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Streptomyces clavuligerus
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme, partial, recombinant enzyme, to near homogenity
Streptomyces clavuligerus
Renatured (Commentary) (protein specific)
Commentary
Organism
enzyme inactivated by 5,5’-dithiobis-2-nitrobenzoic acid, complete reactivation by dithiothreitol
Streptomyces clavuligerus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-exomethylenecephalosporin C + 2-oxoglutarate + O2
-
440380
Streptomyces clavuligerus
deacetylcephalosporin C + succinate + CO2 + H2O
-
-
-
?
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
36
-
-
Streptomyces clavuligerus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
stable up to
Streptomyces clavuligerus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Streptomyces clavuligerus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
9
-
Streptomyces clavuligerus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces clavuligerus
isoelectric focusing
-
5.3
Streptomyces clavuligerus
isoelectric focusing
-
6.1
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744105
Balakrishnan
Modified deacetylcephalospori ...
Streptomyces clavuligerus, Streptomyces clavuligerus ATCC 27064
Appl. Environ. Microbiol.
82
3711-3720
2016
1
-
1
-
53
-
-
5
-
1
-
2
-
3
-
-
-
-
-
-
-
-
4
-
3
1
-
-
6
1
-
-
-
-
-
-
1
-
1
-
-
53
-
-
-
-
5
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
6
1
-
-
-
-
4
4
-
6
6
725639
An
Expression of cefF significant ...
Acremonium chrysogenum, Acremonium chrysogenum CGMCC3.3795, Streptomyces clavuligerus, Streptomyces clavuligerus CGMCC4.1611
J. Ind. Microbiol. Biotechnol.
39
269-274
2012
-
-
2
-
-
-
-
-
-
2
-
4
-
8
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
699366
Goo
Directed evolution and rationa ...
Streptomyces clavuligerus
J. Ind. Microbiol. Biotechnol.
36
619-633
2009
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700004
Liras
Enzymology of beta-lactam comp ...
Actinomyces sp.
Methods Enzymol.
458
401-429
2009
-
1
-
-
-
-
-
3
-
1
-
2
-
1
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671324
Stok
Development of enzyme-linked i ...
Streptomyces clavuligerus
Anal. Chim. Acta
577
153-162
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661796
Wu
C-terminus mutations of Acremo ...
Acremonium chrysogenum
FEMS Microbiol. Lett.
246
103-110
2005
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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1
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-
-
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-
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-
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-
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2
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671443
Wei
Directed evolution of Streptom ...
Streptomyces clavuligerus
Appl. Environ. Microbiol.
71
8873-8880
2005
-
-
1
-
18
-
-
28
-
1
-
1
-
1
-
-
1
-
-
-
-
-
4
-
2
-
-
-
33
-
-
-
-
-
-
-
-
-
1
-
-
18
-
-
-
-
28
-
1
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
33
-
-
-
-
-
-
-
-
-
-
659322
Lloyd
Controlling the substrate sele ...
Acremonium chrysogenum
J. Biol. Chem.
279
15420-15426
2004
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440375
Ghag
Refolding and purification of ...
Acremonium chrysogenum
Biotechnol. Appl. Biochem.
24
109-119
1996
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660772
Coque
Characterization of the cefF g ...
Amycolatopsis lactamdurans
Appl. Microbiol. Biotechnol.
44
605-609
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
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1
1
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1
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-
-
-
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1
-
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-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662088
Baker
Deacetoxycephalosporin C hydro ...
Streptomyces clavuligerus
J. Biol. Chem.
266
5087-5093
1991
2
-
-
-
-
-
7
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
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1
-
-
2
-
-
-
-
-
-
-
7
1
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440380
Dotzlaf
Purification and properties of ...
Streptomyces clavuligerus
J. Biol. Chem.
264
10219-10227
1989
2
-
1
-
-
-
12
-
-
1
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
1
1
-
1
-
1
-
-
2
-
2
-
1
-
-
-
-
-
12
-
-
-
1
1
-
-
-
-
1
1
-
-
-
1
-
-
1
1
-
1
-
1
2
-
-
-
-
-
-
660843
Samson
-
Cloning and expression of the ...
Acremonium chrysogenum
Bio/Technology
5
1207-1214
1987
-
-
1
-
-
-
-
-
-
-
2
-
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1
-
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1
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-
-
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1
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1
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2
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1
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1
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-
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-
-
-
-
-
-
-
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-
660936
Baldwin
Purification and initial chara ...
Acremonium chrysogenum
Biochem. J.
245
831-841
1987
4
-
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1
4
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1
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2
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1
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2
1
1
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1
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4
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1
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4
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1
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1
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2
1
1
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1
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-
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-
661974
Dotzlaf
Copurification and characteriz ...
Acremonium chrysogenum
J. Bacteriol.
169
1611-1618
1987
-
-
-
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8
2
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2
2
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2
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1
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1
1
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1
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1
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1
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8
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2
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2
2
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1
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1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
661955
Jensen
Deacetoxycephalosporin C synth ...
Streptomyces clavuligerus
J. Antibiot.
38
263-265
1985
-
-
-
-
-
-
-
-
1
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1
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2
-
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1
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1
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1
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1
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