BRENDA - Enzyme Database show
show all sequences of 1.14.11.21

Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional a-KG-dependent non-heme iron enzyme: correlation with mchanisms and reactivities

Zhou, J.; Kelly, W.L.; Bachmann, B.O.; Gunsior, M.; Townsend, C.A.; Solomon, E.I.; J. Am. Chem. Soc. 123, 7388-7398 (2001)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
conformation during catalytic reaction; isozyme CS2, a single ferrous active site; isozyme CS2, five and six-coordinate ferrous species in the enzyme-substrate complexes, structural model of conversion for the different reactions; required
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces clavuligerus
Q05581
recombinant purifed isozyme CS2
-
Reaction
Reaction
Commentary
Organism
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces clavuligerus
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O
reaction mechanism
Streptomyces clavuligerus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + O2
in absence or presence of the substrate proclavaminate, inactivation of the enzyme
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
molecular mechanism
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
varying amounts of unsaturated six-coordinate ferrous species exist in the substrate complexes which correlate to this uncoupled reaction
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
amidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639284
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639284
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
additional information
structure-function correlation
639284
Streptomyces clavuligerus
?
-
-
-
-
proclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639284
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
conformation during catalytic reaction; isozyme CS2, a single ferrous active site; isozyme CS2, five and six-coordinate ferrous species in the enzyme-substrate complexes, structural model of conversion for the different reactions; required
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
amidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
guanidinoproclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
clavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
Streptomyces clavuligerus
-
dihydroclavaminate + succinate + CO2 + H2O
-
Streptomyces clavuligerus
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + O2
in absence or presence of the substrate proclavaminate, inactivation of the enzyme
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
molecular mechanism
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
2-oxoglutarate + O2
varying amounts of unsaturated six-coordinate ferrous species exist in the substrate complexes which correlate to this uncoupled reaction
639284
Streptomyces clavuligerus
? + H2O2
-
-
-
?
deoxyamidinoproclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
amidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2
hydroxylation
639284
Streptomyces clavuligerus
guanidinoproclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
dihydroclavaminate + 2-oxoglutarate + O2
cyclization
639284
Streptomyces clavuligerus
clavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
additional information
structure-function correlation
639284
Streptomyces clavuligerus
?
-
-
-
-
proclavaminate + 2-oxoglutarate + O2
-
639284
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
proclavaminate + 2-oxoglutarate + O2
saturation
639284
Streptomyces clavuligerus
dihydroclavaminate + succinate + CO2 + H2O
-
639284
Streptomyces clavuligerus
?
Other publictions for EC 1.14.11.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742159
Shrestha
-
Heterologous production of cl ...
Streptomyces clavuligerus
Biotechnol. Bioprocess Eng.
22
359-365
2017
-
1
1
-
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2
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1
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2
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1
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2
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-
2
-
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-
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-
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-
-
1
1
-
-
-
723902
Chin
-
Predicting the catalytic sites ...
Streptomyces clavuligerus
Afr. J. Microbiol. Res.
5
3357-3366
2011
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-
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1
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2
-
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-
-
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-
-
2
4
-
-
-
685142
Borowski
Mechanism for cyclization reac ...
Streptomyces clavuligerus
Biochemistry
46
3682-3691
2007
-
-
-
1
-
-
-
-
-
-
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1
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1
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1
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-
639036
Townsend
New reactions in clavulanic ac ...
Streptomyces clavuligerus
Curr. Opin. Chem. Biol.
6
583-589
2002
-
-
-
-
-
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1
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4
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1
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1
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9
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1
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9
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-
639286
Zhang
Crystal structure of a clavami ...
Streptomyces clavuligerus
FEBS Lett.
517
7-12
2002
-
-
-
1
-
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-
1
-
4
-
1
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3
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9
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1
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1
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4
-
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-
9
-
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-
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-
-
-
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639284
Zhou
Spectroscopic studies of subst ...
Streptomyces clavuligerus
J. Am. Chem. Soc.
123
7388-7398
2001
-
-
-
-
-
-
-
-
-
1
-
4
-
1
-
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-
2
-
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11
-
-
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-
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-
1
-
4
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-
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-
-
11
-
-
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-
-
-
-
-
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-
-
-
-
639281
Zhang
Structural origins of the sele ...
Streptomyces clavuligerus
Nat. Struct. Biol.
7
127-133
2000
-
-
-
1
-
-
-
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-
1
-
-
-
1
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2
-
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8
-
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-
1
-
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1
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-
-
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-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
639282
Khaleeli
Site-directed mutagenesis and ...
Streptomyces clavuligerus
Biochemistry
39
8666-8673
2000
-
-
1
-
8
-
-
-
-
1
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-
1
-
-
1
-
-
-
-
-
3
-
1
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-
1
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1
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8
-
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-
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-
1
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1
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-
3
-
1
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-
1
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-
-
-
-
639283
Doan
Mutagenesis studies on the iro ...
Streptomyces clavuligerus
Biochem. Biophys. Res. Commun.
279
240-244
2000
-
-
-
-
15
-
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-
1
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1
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2
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-
5
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15
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1
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5
-
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-
639280
Lloyd
-
Product-substrate engineering ...
Streptomyces clavuligerus
Tetrahedron
55
10201-10220
1999
-
-
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1
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4
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1
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3
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11
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1
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11
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639279
Zhou
-
Substrate binding to the alpha ...
Streptomyces clavuligerus
J. Am. Chem. Soc.
120
13539-13540
1998
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1
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1
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9
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639278
Baldwin
-
Chemo-enzymic synthesis of bic ...
Streptomyces clavuligerus
Tetrahedron
53
7011-7020
1997
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1
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639277
Busby
A single monomeric iron center ...
Streptomyces clavuligerus
Bioorg. Med. Chem.
4
1059-1064
1996
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1
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5
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1
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1
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5
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11
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639274
Janc
Purification and characterizat ...
Streptomyces antibioticus, Streptomyces antibioticus Tu1718, Streptomyces clavuligerus
J. Biol. Chem.
270
5399-5404
1995
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3
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2
6
15
-
5
1
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1
6
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1
1
31
2
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2
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3
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2
6
15
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1
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1
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1
1
31
2
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2
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639275
Busby
Expression and purification of ...
Streptomyces clavuligerus
J. Biol. Chem.
270
4262-4269
1995
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1
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1
3
2
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1
2
1
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3
1
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1
2
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2
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6
-
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1
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1
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1
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3
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2
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1
2
1
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1
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1
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2
-
6
-
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1
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639276
Paradkar
Functional analysis of the gen ...
Streptomyces clavuligerus, Streptomyces clavuligerus NRRL 3585
J. Bacteriol.
177
1307-1314
1995
-
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1
-
1
-
-
-
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-
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9
-
5
-
-
-
3
-
-
1
-
12
-
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
-
1
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-
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9
-
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1
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12
-
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-
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639273
Iwata-Reuyl
Synthesis and reaction of pote ...
Streptomyces clavuligerus
J. Nat. Prod.
56
1373-1396
1993
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3
1
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1
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2
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2
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1
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11
-
1
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1
1
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1
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3
1
1
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1
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1
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11
-
1
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1
1
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-
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639272
Elson
-
Studies on the substrate speci ...
Streptomyces clavuligerus
Bioorg. Med. Chem. Lett.
2
1503-1508
1992
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-
-
-
1
-
3
-
1
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639271
Salowe
Elucidation of the order of ox ...
Streptomyces clavuligerus
Biochemistry
30
2281-2292
1991
-
-
-
-
-
-
-
1
-
1
-
3
-
2
-
-
-
3
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639270
Salowe
Purification and characterizat ...
Streptomyces clavuligerus
Biochemistry
29
6499-6508
1990
-
-
-
-
-
-
1
5
-
1
1
3
-
2
1
-
1
2
-
-
1
1
10
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
1
1
3
-
1
-
1
-
-
1
1
10
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-