BRENDA - Enzyme Database show
show all sequences of 1.14.11.20

Isolation and characterization of a 2-oxoglutarate dependent dioxygenase involved in the second-to-last step in vindoline biosynthesis

De Carolis, E.; Chan, F.; Balsevich, J.; De Luca, V.; Plant Physiol. 94, 1323-1329 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbic acid
in vitro required for maximal activity
Catharanthus roseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Catharanthus roseus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
enzyme is induced after exposure of seedlings to light
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
desacetoxyvindoline + 2-oxoglutarate + O2
strictly specific for position 4, no hydroxylation of indole alkaloid substrates with a 2,3-double bond
440241
Catharanthus roseus
desacetylvindoline + succinate + CO2
-
440241
Catharanthus roseus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
absolutely required for activity
Catharanthus roseus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbic acid
in vitro required for maximal activity
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2-oxoglutarate
absolutely required for activity
Catharanthus roseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Catharanthus roseus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
desacetoxyvindoline + 2-oxoglutarate + O2
strictly specific for position 4, no hydroxylation of indole alkaloid substrates with a 2,3-double bond
440241
Catharanthus roseus
desacetylvindoline + succinate + CO2
-
440241
Catharanthus roseus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Catharanthus roseus
Other publictions for EC 1.14.11.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743812
Pandey
Fungal endophytes of Catharan ...
Catharanthus roseus
Sci. Rep.
6
26583
2016
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6
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1
1
1
1
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743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
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1
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2
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726538
Liu
Enhancement of vindoline and v ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2013
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1
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2
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1
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1
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1
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712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
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2
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2
-
3
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3
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2
1
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2
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2
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3
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2
1
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1
1
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684439
Campos-Tamayo
Vindoline formation in shoot c ...
Catharanthus roseus
Ann. Bot.
102
409-415
2008
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3
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2
10
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2
10
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1
1
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660317
Dutta
Expression of terpenoid indole ...
Catharanthus roseus
Planta
220
376-383
2005
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3
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1
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655109
Hernandez-Dominguez
Vindoline synthesis in in vitr ...
Catharanthus roseus
Biotechnol. Lett.
26
671-674
2004
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1
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440245
Vazquez-Flota
Developmental and light regula ...
Catharanthus roseus
Plant Physiol.
117
1351-1361
1998
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1
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2
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1
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440244
Vazquez-Flota
Molecular cloning and characte ...
Catharanthus roseus
Plant Mol. Biol.
34
935-948
1997
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1
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1
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5
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4
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1
1
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1
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1
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4
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1
1
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440243
Carolis
-
A novel 2-oxoglutarate-depende ...
Catharanthus roseus
Plant Cell Tissue Organ Cult.
38
281-287
1994
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440242
De Carolis
Purification, characterization ...
Catharanthus roseus
J. Biol. Chem.
268
5504-5511
1993
1
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5
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2
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2
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2
5
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440241
De Carolis
Isolation and characterization ...
Catharanthus roseus
Plant Physiol.
94
1323-1329
1990
1
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