Protein Variants | Comment | Organism |
---|---|---|
H131A/D315A | inactive mutant | Homo sapiens |
additional information | transient transfection of HeLa and A549 cells with siRNA-targeting PHD2 or PHD3, transient downregulation of PHD3 in HeLa cells or transfection of the control siRNA does not affect the phosphorylation of Cof-1, downregulation of PHD2 leads to an increase of phosphorylated Cof-1. Induction of a PHD2 knockdown in tetracycline-inducible HeLa PHD2 knockdown cells, 1B6 and 3B7 cells from cell line 2.1.1-16, results in increased F-actin formation as detected by phalloidin staining. PHD2 knockdown impairs cell migration. Wild-type PHD2 reverses the increased p-Cof1 levels in 3B7 cells, whereas the mutant PHD2 has no similar effect | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
prolyl hydroxylase | - |
Homo sapiens |
prolyl-4-hydroxylase | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | PHD2 is hypoxia-inducible by HIF-1alpha | up |
General Information | Comment | Organism |
---|---|---|
physiological function | PHD2 mediates F-actin assembly involving inhibition of Cof-1 phosphorylation. Isozyme PHD2 is the main isoform regulating HIF-1alpha hydroxylation and thus stability in normoxia | Homo sapiens |