Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.2 extracted from

  • Hieta, R.; Myllyharju, J.
    Cloning and characterization of a low molecular weight prolyl 4-hydroxylase from Arabidopsis thaliana. Effective hydroxylation of proline-rich, collagen-like, and hypoxia-inducible transcription factor a-like peptides (2002), J. Biol. Chem., 277, 23965-23971.
    View publication on PubMed
Search for more literature for 1.14.11.2

Cloned(Commentary)

Cloned (Comment) Organism
the genome encodes six enzyme-like polypeptides, that show an identity of 21-27% to the catalitically important C-terminal regions of the human enzyme alpha-I and alpha-II subunits. Cloning of one of the genes, recombinant enzyme expressed in insect cells and in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H180A the mutation completely inactivates the enzyme Arabidopsis thaliana
H180Q the mutation completely inactivates the enzyme Arabidopsis thaliana
H260A the mutation completely inactivates the enzyme Arabidopsis thaliana
H260Q the mutation completely inactivates the enzyme Arabidopsis thaliana
K270A the mutation completely inactivates the enzyme Arabidopsis thaliana
K270R the mutation completely inactivates the enzyme Arabidopsis thaliana
N182A the mutation completely inactivates the enzyme Arabidopsis thaliana
N182Q the mutation completely inactivates the enzyme Arabidopsis thaliana
R278A the mutation completely inactivates the enzyme Arabidopsis thaliana
R278H the mutation reduces the enzyme activity to approximately 26% Arabidopsis thaliana
S272A the mutation reduces the enzyme activity by 83% Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km values of enzyme for peptides representing transcription factor HIF alpha sequences and containing only one proline residue Arabidopsis thaliana
0.0002
-
 poly(L-proline)
-
 Arabidopsis thaliana
0.0002
-
 poly(L-proline) MW: 10000-20000 Arabidopsis thaliana
0.0002
-
 poly(L-proline) isoform P4H1 Arabidopsis thaliana
0.0002
-
 poly(L-proline) isoform P4H4 Arabidopsis thaliana
0.0002
-
 poly(L-proline) isoform P4H7 Arabidopsis thaliana
0.002
-
 poly(L-proline)
-
 Arabidopsis thaliana
0.002
-
 (Pro-Ala-Pro-Lys)10
-
 Arabidopsis thaliana
0.002
-
 poly(L-proline) MW: 5000-10000 Arabidopsis thaliana
0.002
-
 poly(L-proline) isoform P4H1 Arabidopsis thaliana
0.002
-
 poly(L-proline) isoform P4H4 Arabidopsis thaliana
0.002
-
 poly(L-proline) isoform P4H7 Arabidopsis thaliana
0.004
-
 (Pro-Glu-Pro-Pro-Ala)5
-
 Arabidopsis thaliana
0.01
-
 Ser-Pro-Pro-Pro-Pro-Val-Ser-Pro-Pro-Pro-Val-Ser-Pro-Pro-Pro-Pro-Val
-
 Arabidopsis thaliana
0.02
-
 (Ser-Pro-Lys-Pro-Pro)5
-
 Arabidopsis thaliana
0.02
-
 (Ala-Thr-Pro-Pro-Pro-Val)3
-
 Arabidopsis thaliana
0.04
-
 Ser-Pro-Pro-Pro-Val-Tyr-Lys-Ser-Pro-Pro-Pro-Pro-Val-Lys-His-Tyr-Ser-Pro-Pro-Pro-Val
-
 Arabidopsis thaliana
0.06
-
 (Pro-Pro-Gly)10
-
 Arabidopsis thaliana
0.06
-
 (L-Pro-L-Pro-Gly)10 isoform P4H1 Arabidopsis thaliana
0.06
-
 (L-Pro-L-Pro-Gly)10 isoform P4H4 Arabidopsis thaliana
0.06
-
 (L-Pro-L-Pro-Gly)10 isoform P4H7 Arabidopsis thaliana
0.09
-
 (Pro-Ala-Pro-Lys)3
-
 Arabidopsis thaliana
0.09
-
 (L-Pro-L-Ala-L-Pro-L-Lys)3 isoform P4H1 Arabidopsis thaliana
0.09
-
 (L-Pro-L-Ala-L-Pro-L-Lys)3 isoform P4H4 Arabidopsis thaliana
0.09
-
 (L-Pro-L-Ala-L-Pro-L-Lys)3 isoform P4H7 Arabidopsis thaliana
0.1
-
 (Ala-Pro-Gly)5
-
 Arabidopsis thaliana
0.12
-
 (Pro-Pro-Gly)5
-
 Arabidopsis thaliana
0.12
-
 (L-Pro-L-Pro-Gly)5 isoform P4H1 Arabidopsis thaliana
0.12
-
 (L-Pro-L-Pro-Gly)5 isoform P4H4 Arabidopsis thaliana
0.12
-
 (L-Pro-L-Pro-Gly)5 isoform P4H7 Arabidopsis thaliana
0.13
-
 2-oxoglutarate
-
 Arabidopsis thaliana
0.28
-
 (Pro-Ala-Gly)5
-
 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Km: 0.016 mM Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Q8L970
-
-
Arabidopsis thaliana Q8LAN3
-
-
Arabidopsis thaliana Q9ZW86
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ala-Pro-Gly)5 + 2-oxoglutarate + O2 recombinant enzyme Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Ala-Thr-Pro-Pro-Pro-Val)3 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Gly-Pro-4Hyp)5 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(L-Pro-L-Ala-L-Pro-L-Lys)3 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana (L-Pro-L-Ala-L-Pro-L-Lys)3-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
(Pro-Ala-Gly)5 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Pro-Ala-Pro-Lys)n + 2-oxoglutarate + O2 n: 3,10 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Pro-Glu-Pro-Pro-Ala)5 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Arabidopsis thaliana (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 the enzyme acts in (Pro-Pro-Gly)10 preferentially on prolines in Y positions in the X-Y-Gly triplets Arabidopsis thaliana (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Ser-Pro-Lys-Pro-Pro)5 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
Asp-Ala-Leu-Thr-Leu-Leu-Ala-Pro-Ala-Ala-Gly-Asp-Thr-Ile-Ile-Ser-Leu-Phe-Gly + 2-oxoglutarate + O2 peptide representing hypoxia-inducible transcription factor alpha sequences Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
Asp-Leu-Asp-Leu-Glu-Met-Leu-Ala-Pro-Tyr-Ile-Pro-Met-Asp-Asp-Asp-Phe-Gln-Leu + 2-oxoglutarate + O2 peptide representing hypoxia-inducible transcription factor alpha sequences Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
additional information the enzyme preferentially hydroxylates proline residues preceding glycines in (X-Y-Gly)n peptides. Peptides representing the N- and C-terminal hydroxylation sites present in hypoxia-inducible transcription factor alpha also serve as substrates Arabidopsis thaliana ?
-
 ?
poly(L-Pro) + 2-oxoglutarate + O2 recombinant enzyme Arabidopsis thaliana poly(4-hydroxyproline) + succinate + CO2
-
 ?
poly(L-proline) + 2-oxoglutarate + O2 poly(L-proline) with Mr 10000-2000 Arabidopsis thaliana poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
poly(L-proline) + 2-oxoglutarate + O2 poly(L-proline) with Mr 5000-1000 Arabidopsis thaliana poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r
Ser-Pro-Pro-Pro-Pro-Val-Ser-Pro-Pro-Pro-Val-Ser-Pro-Pro-Pro-Pro-Val + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
Ser-Pro-Pro-Pro-Val-Tyr-Lys-Ser-Pro-Pro-Pro-Pro-Val-Lys-His-Tyr-Ser-Pro-Pro-Pro-Val + 2-oxoglutarate + O2
-
 Arabidopsis thaliana 4-hydroxyproline containing peptide + succinate + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ascorbate recombinant enzyme: Km 0.3 mM Arabidopsis thaliana