Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the genome of virus-1 encodes a 242-amino acid polypeptide that shows a distinct amino acid sequence similarity to the C-terminal half of the catalytic alpha subunit of animal enzymes, the genome contains many open reading frames for proteins with proline-rich repeats | Paramecium bursaria Chlorella virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-oxoglutarate | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.02 | - |
(Pro-Ala-Pro-Lys)10 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.02 | - |
(Ser-Pro-Lys-Pro-Pro)5 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.05 | - |
(Pro-Ala-Pro-Lys)5 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.1 | - |
poly(L-proline) | MW: 40000, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.31 | - |
(L-Pro-L-Ala-L-Pro-L-Lys)3 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.5 | - |
poly(L-proline) | MW: 13000, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
0.95 | - |
(L-Pro-L-Ala-L-Pro-L-Lys)2 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
1 | - |
(Pro-Glu-Pro-Pro-Ala)5 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
2.9 | - |
(L-Pro-L-Pro-Gly)10 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
4.8 | - |
L-Pro-L-Ala-L-Pro-L-Lys | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
8.6 | - |
Lys-Pro-Ala | recombinant enzyme | Paramecium bursaria Chlorella virus 1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | recombinant enzyme: Km 0.0004 mM | Paramecium bursaria Chlorella virus 1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27200 | - |
recombinant enzyme, calculated with the N-terminal histidine tag and the thrombin cleavage site | Paramecium bursaria Chlorella virus 1 |
30000 | - |
recombinant enzyme, gel filtration | Paramecium bursaria Chlorella virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus 1 | - |
virus-1, eukaryotic algal virus | - |
Purification (Comment) | Organism |
---|---|
using Ni2+-chelate affinity chromatography | Paramecium bursaria Chlorella virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Ala-L-Pro-L-Lys)2 + 2-oxoglutarate + O2 | - |
Paramecium bursaria Chlorella virus 1 | (L-Pro-L-Ala-L-Pro-L-Lys)2-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(L-Pro-L-Ala-L-Pro-L-Lys)3 + 2-oxoglutarate + O2 | - |
Paramecium bursaria Chlorella virus 1 | (L-Pro-L-Ala-L-Pro-L-Lys)3-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Paramecium bursaria Chlorella virus 1 | (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(Pro-Ala-Pro-Lys)n + 2-oxoglutarate + O2 | n: 1-10, recombinant enzyme, (Pro-Ala-Pro-Lys)10 is a particularly good substrate | Paramecium bursaria Chlorella virus 1 | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Pro-Glu-Pro-Pro-Ala)5 + 2-oxoglutarate + O2 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Paramecium bursaria Chlorella virus 1 | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
(Ser-Pro-Lys-Pro-Pro)5 + 2-oxoglutarate + O2 | recombinant enzyme, (Ser-Pro-Lys-Pro)5 is a particularly good substrate | Paramecium bursaria Chlorella virus 1 | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
L-Pro-L-Ala-L-Pro-L-Lys + 2-oxoglutarate + O2 | - |
Paramecium bursaria Chlorella virus 1 | L-Pro-L-Ala-L-Pro-L-Lys-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
Lys-Pro-Ala + 2-oxoglutarate + O2 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | Lys-4-hydroxyproline-Ala + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | recombinant enzyme | Paramecium bursaria Chlorella virus 1 | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-proline) + 2-oxoglutarate + O2 | poly(L-proline) with Mr 40000 | Paramecium bursaria Chlorella virus 1 | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
r | |
poly(L-proline) + 2-oxoglutarate + O2 | poly(L-proline) with Mr 13000 | Paramecium bursaria Chlorella virus 1 | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | recombinant enzyme, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Paramecium bursaria Chlorella virus 1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | recombinant enzyme: Km 0.3 mM | Paramecium bursaria Chlorella virus 1 |