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Literature summary for 1.14.11.2 extracted from

  • Helaakoski, T.; Annunen, P.; Vuori, K.; MacNeil, I.A.; Pihlajaniemi, T.; Kivirikko, K.I.
    Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase a-subunit isoform: formation of an a2b2 tetramer with the protein disulfide-isomerase/b subunit (1995), Proc. Natl. Acad. Sci. USA, 92, 4427-4431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the alpha-I subunit and of a second mouse alpha-subunit isoform, termed the alpha-II subunit, expression of the alpha-II subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
poly(L-proline) inhibitory, type I enzyme tetramer, MW: 7000 and 44000 Homo sapiens
poly(L-proline) inhibitor, recombinant type II enzyme tetramer, MW: 7000 and 44000 Mus musculus
Pyridine 2,4-dicarboxylate inhibitory, type I enzyme tetramer Homo sapiens
Pyridine 2,4-dicarboxylate inhibitory, recombinant type II enzyme tetramer Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
2-oxoglutarate recombinant type II enzyme tetramer Mus musculus
0.018
-
(Pro-Pro-Gly)10 type I enzyme tetramer Homo sapiens
0.022
-
2-oxoglutarate type I enzyme tetramer Homo sapiens
0.045
-
(L-Pro-L-Pro-Gly)10 recombinant type II enzyme tetramer Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ recombinant type II enzyme tetramer: Km 0.004 mM Mus musculus
Fe2+ type I enzyme tetramer: Km 0.004 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
brain expression of alpha-I and alpha-II subunits mRNAs Mus musculus
-
heart
-
Homo sapiens
-
heart expression of enzyme alpha-I and alpha-II subunits mRNAs Mus musculus
-
lung
-
Homo sapiens
-
lung expression of alpha-I and alpha-II subunits mRNAs Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
Mus musculus (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
r
(Pro-Pro-Gly)10 + 2-oxoglutarate + O2
-
Homo sapiens ? + succinate + CO2
-
?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Mus musculus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Homo sapiens (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Cofactor

Cofactor Comment Organism Structure
ascorbate recombinant type II enzyme tetramer: Km 0.34 mM Mus musculus
ascorbate type I enzyme tetramer: Km 0.33 mM Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
poly (L-proline) MW: 44000, type I enzyme tetramer Homo sapiens
0.0005
-
poly (L-proline) MW: 7000, type I enzyme tetramer Homo sapiens
0.001
-
Pyridine 2,4-dicarboxylate
-
Mus musculus
0.001
-
Pyridine 2,4-dicarboxylate recombinant type II enzyme tetramer Homo sapiens
0.002
-
Pyridine 2,4-dicarboxylate type I enzyme tetramer Homo sapiens
0.03
-
poly (L-proline)
-
Mus musculus
0.03
-
poly (L-proline) MW: 44000, recombinant type II enzyme tetramer Homo sapiens
0.3
-
poly (L-proline)
-
Mus musculus
0.3
-
poly (L-proline) MW: 7000, recombinant type II enzyme tetramer Homo sapiens