Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Gallus gallus | |
catalase | activation | Gallus gallus | |
dithiothreitol | activation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethyloxalylalanine | 50% inhibition in chicken embryo calvaria at 1 mM | Gallus gallus | |
dimethyloxalylglycine | 50% inhibition in chicken embryo calvaria at 0.002 mM, inhibitor of hydroxyproline synthesis in embryonic chicken lung | Gallus gallus | |
oxalyl-beta-alanine | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
oxalylalanine | inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.123 mM | Gallus gallus | |
oxalylcystine | competitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
oxalylglycine | inhibits purified enzyme, competitive inhibition with respect to 2-oxoglutarate, 50% inhibition of microsomal enzyme at 0.023 mM | Gallus gallus | |
oxalylproline | - |
Gallus gallus | |
oxalylsarcosine | noncompetitive inhibition with respect to 2-oxoglutarate | Gallus gallus | |
oxalylvaline | - |
Gallus gallus | |
Pyridine 2,4-dicarboxylate | - |
Gallus gallus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | embryonic bone | Gallus gallus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, affinity chromatography on poly-L-proline coupled to Sepharose 4B and DEAE-cellulose chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | embryo | Gallus gallus | - |
calvarium | embryo | Gallus gallus | - |
embryo | - |
Gallus gallus | - |
lung | embryo | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 1,5,10 | Gallus gallus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | n: 1,5,10 | ? | |
additional information | thermal denaturing of the triple-helical conformation of the substrate before hydroxylation | Gallus gallus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | 0.007 | oxalylglycine | - |
Gallus gallus | |
0.04 | - |
oxalylalanine | - |
Gallus gallus | |
1 | - |
oxalylsarcosine | - |
Gallus gallus | |
1.25 | - |
oxalylcystine | - |
Gallus gallus | |
1.4 | - |
oxalyl-beta-alanine | - |
Gallus gallus | |
10 | - |
oxalylproline | value above | Gallus gallus | |
10 | - |
oxalylvaline | value above | Gallus gallus |