Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activation | Catharanthus roseus | |
dithiothreitol | activation | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | high concentrations of salts are inhibitory | Catharanthus roseus | |
NaCl | more than 0.3 M | Catharanthus roseus | |
poly-L-hydroxyproline | MW: 30000, inhibitory | Catharanthus roseus | |
potassium phosphate | - |
Catharanthus roseus | |
Sodium acetate | - |
Catharanthus roseus | |
succinate | 11.5% inhibition at 0.5 mM and 38.3% inhibition at 3 mM | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
O2 | - |
Catharanthus roseus | |
0.23 | - |
poly(L-proline) | - |
Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | 40.7% of total activity | Catharanthus roseus | - |
- |
mitochondrion | 47.9% of total activity | Catharanthus roseus | 5739 | - |
soluble | 11.4% of total activity | Catharanthus roseus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Catharanthus roseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Catharanthus roseus | - |
Storage Stability | Organism |
---|---|
-80°C, dialyzed against 25% glycerol, 1 month | Catharanthus roseus |
4°C, unstable | Catharanthus roseus |
freeze-thawing, unstable | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 | n: 5,10, poor substrates | Catharanthus roseus | (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 | - |
? | |
Gly-Pro8 + 2-oxoglutarate + O2 | 96% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? | |
additional information | no reaction with: free proline, prolyl peptides whose residues number is four or less | Catharanthus roseus | ? | - |
? | |
octa-L-proline + 2-oxoglutarate + O2 | 81.8% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | MW 2000, 6000 and 12000 | Catharanthus roseus | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-proline) + 2-oxoglutarate + O2 | - |
Catharanthus roseus | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
r | |
tert-butyloxycarbonyl-Pro8 + 2-oxoglutarate + O2 | 91.4% relative activity with respect to poly(L-proline) of MW 2000 | Catharanthus roseus | 4-hydroxyproline containing peptide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | Km: 0.09 mM | Catharanthus roseus | |
ascorbate | - |
Catharanthus roseus | |
additional information | alpha-ketoglutarate cannot be replaced by pyruvate and oxaloacetate | Catharanthus roseus |