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Literature summary for 1.14.11.2 extracted from
- Recent developments in posttranslational modification: intracellular processing (1987), Methods Enzymol., 144, 96-114.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | activation | Gallus gallus |  |
| cysteine | activation | Homo sapiens | |
| dithiothreitol | activation | Gallus gallus | |
| dithiothreitol | activation | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| preparation of cDNA clones for the two subunits, expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dihydroxybenzoate | - |
Gallus gallus | |
| 2,3-Dihydroxybenzoate | - |
Homo sapiens | |
| 3,4-dihydroxybenzoate | - |
Gallus gallus | |
| 3,4-dihydroxybenzoate | - |
Homo sapiens | |
| 3,4-Dihydroxyphenylacetate | - |
Gallus gallus | |
| 3,4-Dihydroxyphenylacetate | - |
Homo sapiens | |
| 4-hydroxybenzoate | - |
Gallus gallus | |
| 4-hydroxybenzoate | - |
Homo sapiens | |
| Cu2+ | - |
Gallus gallus | |
| Cu2+ | - |
Homo sapiens | |
| glutamyl-3,4-dehydroprolyl-bradykinin | - |
Gallus gallus | |
| glutamyl-3,4-dehydroprolyl-bradykinin | - |
Homo sapiens | |
| hydroxybenzene | - |
Gallus gallus | |
| poly(L-proline) | - |
Gallus gallus | |
| poly(L-proline) | - |
Homo sapiens | |
| poly(L-proline) | - |
Mus musculus | |
| Pyridine 2,4-dicarboxylate | - |
Gallus gallus | |
| Pyridine 2,4-dicarboxylate | - |
Homo sapiens | |
| Pyridine 2,4-dicarboxylate | - |
Mus musculus | |
| Pyridine 2,5-dicarboxylate | - |
Gallus gallus | |
| Pyridine 2,5-dicarboxylate | - |
Homo sapiens | |
| pyridine 2-carboxylate | - |
Gallus gallus | |
| Zn2+ | - |
Gallus gallus | |
| Zn2+ | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Gallus gallus | |
| Fe2+ | - |
Mus musculus | |
| Fe2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate composition | Gallus gallus |
| glycoprotein | carbohydrate composition | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of subunits | Gallus gallus |
| using affinity chromatography, ion-exchange chromatography and gel filtration | Gallus gallus |
| using affinity chromatography, ion-exchange chromatography and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Gallus gallus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| proline containing peptide + 2-oxoglutarate + O2 | the presence of 2-oxoglutarate is an absolute and highly specific requirement, the formation of 4-hydroxyproline is accompanied by a stoichiometric decarboxylation of 2-oxoglutarate, the oxygen of the hydroxyl group is derived from molecular oxygen, the other atom of the O2 molecule being incorporated into the succinate, the activated form of oxygen is probably superoxide | Gallus gallus | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
| proline containing peptide + 2-oxoglutarate + O2 | the presence of 2-oxoglutarate is an absolute and highly specific requirement, the formation of 4-hydroxyproline is accompanied by a stoichiometric decarboxylation of 2-oxoglutarate, the oxygen of the hydroxyl group is derived from molecular oxygen, the other atom of the O2 molecule being incorporated into the succinate, the activated form of oxygen is probably superoxide | Homo sapiens | 4-hydroxyproline containing peptide + succinate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | fibroblasts, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms | Gallus gallus |
| tetramer | newborn, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms | Mus musculus |
| tetramer | liver, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms | Homo sapiens |
| tetramer | alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE | Gallus gallus |
| tetramer | alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE | Homo sapiens |
| tetramer | there are two forms of the alpha subunit | Gallus gallus |
| tetramer | there are two forms of the alpha subunit | Mus musculus |
| tetramer | there are two forms of the alpha subunit | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | - |
Mus musculus | |
| ascorbate | highly specific for | Gallus gallus | |
| ascorbate | highly specific for | Homo sapiens | |
| ascorbate | oxygen acceptor in the decarboxylation of 2-oxoglutarate without subsequent hydroxylation of peptide substrate | Gallus gallus | |
| ascorbate | oxygen acceptor in the decarboxylation of 2-oxoglutarate without subsequent hydroxylation of peptide substrate | Homo sapiens | |
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