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Literature summary for 1.14.11.16 extracted from

  • Brewitz, L.; Tumber, A.; Schofield, C.J.
    Kinetic parameters of human aspartate/asparagine-beta-hydroxylase suggest that it has a possible function in oxygen sensing (2020), J. Biol. Chem., 295, 7826-7838 .
    View publication on PubMed
Search for more literature for 1.14.11.16

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 2-oxoglutarate pH 7.5, 20°C Homo sapiens
0.0012
-
 [thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate pH 7.5, 20°C Homo sapiens
0.426
-
 O2 pH 7.5, 20°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-aspartate + 2-oxoglutarate + O2 Homo sapiens the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains peptide-3-hydroxy-L-aspartate + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q12797
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptide-L-aspartate + 2-oxoglutarate + O2 the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains Homo sapiens peptide-3-hydroxy-L-aspartate + succinate + CO2
-
 ?
[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate + 2-oxoglutarate + O2 the enzyme accepts substrates with a noncanonical EGFD disulfide connectivity (i.e. the Cys 1-2, 3-4, 5-6 disulfide pattern). Stable cyclic thioether analogues of the noncanonical EGFD AspH substrates are developed to avoid disulfide shuffling Homo sapiens [thioether-linked cyclic peptide hFX-CP101-119]-3-hydroxy-L-aspartate + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
aspartate/asparagine-beta-hydroxylase
-
 Homo sapiens
ASPH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 2-oxoglutarate pH 7.5, 20°C Homo sapiens
0.2
-
 [thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate pH 7.5, 20°C Homo sapiens
0.23
-
 O2 pH 7.5, 20°C Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.54
-
 O2 pH 7.5, 20°C Homo sapiens
170
-
 [thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate pH 7.5, 20°C Homo sapiens
320
-
 2-oxoglutarate pH 7.5, 20°C Homo sapiens