Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
2-oxoglutarate | pH 7.5, 20°C | Homo sapiens | |
0.0012 | - |
[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate | pH 7.5, 20°C | Homo sapiens | |
0.426 | - |
O2 | pH 7.5, 20°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-aspartate + 2-oxoglutarate + O2 | Homo sapiens | the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains | peptide-3-hydroxy-L-aspartate + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q12797 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-aspartate + 2-oxoglutarate + O2 | the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains | Homo sapiens | peptide-3-hydroxy-L-aspartate + succinate + CO2 | - |
? | |
[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate + 2-oxoglutarate + O2 | the enzyme accepts substrates with a noncanonical EGFD disulfide connectivity (i.e. the Cys 1-2, 3-4, 5-6 disulfide pattern). Stable cyclic thioether analogues of the noncanonical EGFD AspH substrates are developed to avoid disulfide shuffling | Homo sapiens | [thioether-linked cyclic peptide hFX-CP101-119]-3-hydroxy-L-aspartate + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate/asparagine-beta-hydroxylase | - |
Homo sapiens |
ASPH | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
2-oxoglutarate | pH 7.5, 20°C | Homo sapiens | |
0.2 | - |
[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate | pH 7.5, 20°C | Homo sapiens | |
0.23 | - |
O2 | pH 7.5, 20°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
O2 | pH 7.5, 20°C | Homo sapiens | |
170 | - |
[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate | pH 7.5, 20°C | Homo sapiens | |
320 | - |
2-oxoglutarate | pH 7.5, 20°C | Homo sapiens |