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Literature summary for 1.14.11.16 extracted from

  • Koivunen, P.; Hirsilae, M.; Guenzler, V.; Kivirikko, K.I.; Myllyharju, J.
    Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases (2004), J. Biol. Chem., 279, 9899-9904.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells as His-tag and GST-fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information several 2-oxoglutarate analogs inhibits enzyme activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
2-oxoglutarate pH 7.8, 30°C Homo sapiens
0.09
-
O2 pH 7.8, 30°C Homo sapiens
0.1
-
HIF-1alpha peptide Asp788-Leu822 (L-asparagine803) pH 7.8, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide L-asparagine + 2-oxoglutarate + O2 Homo sapiens hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 peptide 3-hydroxy-L-asparagine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NWT6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
HIF-1alpha peptide Asp788-Leu822 (L-Asn803) + 2-oxoglutarate + O2 much lower activity with peptides lacking residues 819-822, 807-822, and 815-822 Homo sapiens HIF-1alpha peptide Asp788-Leu822 (3-hydroxy-L-Asn803) + succinate + CO2
-
?
HIF-1alpha peptide Asp788-Leu822 (L-asparagine803) + 2-oxoglutarate + O2
-
Homo sapiens ?
-
r
peptide L-asparagine + 2-oxoglutarate + O2
-
Homo sapiens peptide 3-hydroxy-L-asparagine + succinate + CO2
-
?
peptide L-asparagine + 2-oxoglutarate + O2 hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 Homo sapiens peptide 3-hydroxy-L-asparagine + succinate + CO2
-
?

Subunits

Subunits Comment Organism
dimer homodimer Homo sapiens

Synonyms

Synonyms Comment Organism
factor inhibiting hypoxia-inducible transcription factor (HIF)
-
Homo sapiens
FIH
-
Homo sapiens