Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells as His-tag and GST-fusion protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | several 2-oxoglutarate analogs inhibits enzyme activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
2-oxoglutarate | pH 7.8, 30°C | Homo sapiens | |
0.09 | - |
O2 | pH 7.8, 30°C | Homo sapiens | |
0.1 | - |
HIF-1alpha peptide Asp788-Leu822 (L-asparagine803) | pH 7.8, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide L-asparagine + 2-oxoglutarate + O2 | Homo sapiens | hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NWT6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
HIF-1alpha peptide Asp788-Leu822 (L-Asn803) + 2-oxoglutarate + O2 | much lower activity with peptides lacking residues 819-822, 807-822, and 815-822 | Homo sapiens | HIF-1alpha peptide Asp788-Leu822 (3-hydroxy-L-Asn803) + succinate + CO2 | - |
? | |
HIF-1alpha peptide Asp788-Leu822 (L-asparagine803) + 2-oxoglutarate + O2 | - |
Homo sapiens | ? | - |
r | |
peptide L-asparagine + 2-oxoglutarate + O2 | - |
Homo sapiens | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? | |
peptide L-asparagine + 2-oxoglutarate + O2 | hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300 | Homo sapiens | peptide 3-hydroxy-L-asparagine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
factor inhibiting hypoxia-inducible transcription factor (HIF) | - |
Homo sapiens |
FIH | - |
Homo sapiens |