BRENDA - Enzyme Database show
show all sequences of 1.14.11.16

Partial purification and characterization of bovine liver aspartyl beta-hydroxylase

Gronke, R.S.; Welsch, D.J.; VanDusen, W.J.; Garsky, V.M.; Sardana, M.K.; Stern, A.M.; Friedman, P.A.; J. Biol. Chem. 265, 8558-8565 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
Fe2+
-
Bos taurus
0.005
-
2-oxoglutarate
-
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
rough endoplasmic reticulum
-
Bos taurus
5791
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
peptide L-aspartate + 2-oxoglutarate + O2
first epidermal growth factor-like domain of human protein S as substrate
439259
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439259
Bos taurus
?
peptide L-aspartate + 2-oxoglutarate + O2
specific erythro-hydroxylation
439259
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439259
Bos taurus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
7.9
about half-maximal activity at pH 6.5 and 7.9
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
Fe2+
-
Bos taurus
0.005
-
2-oxoglutarate
-
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
rough endoplasmic reticulum
-
Bos taurus
5791
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
peptide L-aspartate + 2-oxoglutarate + O2
first epidermal growth factor-like domain of human protein S as substrate
439259
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439259
Bos taurus
?
peptide L-aspartate + 2-oxoglutarate + O2
specific erythro-hydroxylation
439259
Bos taurus
peptide 3-hydroxy-L-aspartate + succinate + CO2
-
439259
Bos taurus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
7.9
about half-maximal activity at pH 6.5 and 7.9
Bos taurus
Other publictions for EC 1.14.11.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743376
Tang
Aspartate beta-hydroxylase di ...
Homo sapiens
Oncogenesis
6
e362
2017
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1
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1
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-
743379
Ma
Prolyl hydroxylase domain pro ...
Homo sapiens
Oncotarget
8
12983-13002
2017
-
-
-
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-
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-
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2
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1
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1
3
3
1
-
-
742626
Iwagami
Aspartate beta-hydroxylase mo ...
Homo sapiens
Hepatology
63
1213-1226
2016
-
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1
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1
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2
2
-
-
-
742226
Lyu
-
Research progress of aspartyl ...
Homo sapiens
Cancer Res. Clinic
27
280-282
2015
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-
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1
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1
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2
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-
1
2
2
1
-
-
742572
Yang
The aspartyl (asparaginyl) be ...
Homo sapiens
Front. Biosci.
20
902-909
2015
-
-
-
-
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2
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2
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2
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1
1
1
1
-
-
743130
Borgas
Regulation of aspartyl-(aspar ...
Homo sapiens
J. Nat. Sci.
1
e84
2015
-
1
-
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1
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1
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1
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1
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1
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1
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1
-
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1
-
-
743377
Dong
Aspartate beta-hydroxylase ex ...
Homo sapiens
Oncotarget
6
1231-1248
2015
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
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1
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1
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1
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-
-
-
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1
2
2
1
-
-
743882
Tomimaru
Aspartate-beta-hydroxylase in ...
Homo sapiens
Vaccine
33
1256-1266
2015
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
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1
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1
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-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
742666
Huyan
Inhibition of human natural k ...
Homo sapiens
Int. Immunopharmacol.
23
452-459
2014
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
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2
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2
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2
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-
1
1
-
-
-
725118
Yang
Single-chain variable fragment ...
Homo sapiens
Hybridoma
30
69-79
2011
-
-
-
-
-
-
1
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
1
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1
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1
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1
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2
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1
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-
-
-
695432
de la Monte
Ethanol inhibition of aspartyl ...
Rattus norvegicus
Alcohol
43
225-240
2009
1
-
1
-
-
-
2
-
-
-
2
-
-
3
-
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-
-
-
3
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1
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1
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2
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2
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3
-
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2
2
2
2
-
-
698158
Longato
Overexpression of insulin rece ...
Mus musculus
Hepatology
49
1935-1943
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
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-
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-
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-
-
-
-
-
1
1
1
1
-
-
698195
Luu
Prognostic value of aspartyl ( ...
Homo sapiens
Hum. Pathol.
40
639-644
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
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-
-
1
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
698201
Xue
Monoclonal antibodies against ...
Homo sapiens
Hybridoma
28
251-257
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
-
-
-
-
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1
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1
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1
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1
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-
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-
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-
-
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-
-
-
-
699589
Hardy
Asparaginyl beta-hydroxylation ...
Homo sapiens
J. Mol. Biol.
392
994-1006
2009
-
-
1
-
-
-
-
-
-
-
-
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2
-
-
1
-
-
-
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1
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1
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1
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1
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1
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1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
684228
Carter
Ethanol impaired neuronal migr ...
Homo sapiens
Acta Neuropathol.
116
303-315
2008
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
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1
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1
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1
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1
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1
-
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-
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-
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-
673951
Gundogan
Role of aspartyl-(asparaginyl) ...
Homo sapiens
Hum. Pathol.
38
50-59
2007
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
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-
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4
-
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4
-
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-
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-
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-
687022
Yeung
Isolation and characterization ...
Homo sapiens
Hum. Antibodies
16
163-176
2007
-
2
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
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-
-
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2
1
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687592
Coleman
Asparaginyl hydroxylation of t ...
Homo sapiens, Mus musculus
J. Biol. Chem.
282
24027-24038
2007
-
-
-
2
-
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2
-
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2
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2
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2
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2
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2
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673915
Cantarini
Aspartyl-asparagyl beta hydrox ...
Homo sapiens
Hepatology
44
446-457
2006
-
-
-
-
-
-
-
-
-
-
-
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2
-
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1
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1
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1
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1
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675183
de la Monte
Aspartyl-(asparaginyl)-beta-hy ...
Homo sapiens
J. Hepatol.
44
971-983
2006
-
-
1
-
-
-
-
-
-
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1
-
-
3
-
-
-
-
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3
-
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1
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1
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1
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3
-
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1
-
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-
-
-
-
-
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-
-
-
-
-
-
675861
Xian
Expression of aspartyl beta-hy ...
Homo sapiens
Mod. Pathol.
19
280-286
2006
-
-
-
-
-
-
-
-
-
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2
-
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-
-
-
2
-
-
1
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2
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
676817
Cockman
Posttranslational hydroxylatio ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
14767-14772
2006
-
-
-
-
-
-
1
-
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-
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2
-
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1
-
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1
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1
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1
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1
-
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-
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-
-
-
-
-
-
-
-
-
657901
Lancaster
Factor inhibiting hypoxia-indu ...
Homo sapiens
Biochem. Soc. Trans.
32
943-945
2004
-
-
-
-
-
-
-
-
-
1
-
2
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