Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Capsicum annuum | |
ascorbate | required | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene GA4, expression as maltose-binding protein-fusion protein in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydrocinnamic acid | - |
Arabidopsis thaliana | |
Hydrocinnamic acid | - |
Capsicum annuum | |
indole acetic acid | - |
Arabidopsis thaliana | |
indole acetic acid | - |
Capsicum annuum | |
indole propionic acid | - |
Arabidopsis thaliana | |
indole propionic acid | - |
Capsicum annuum | |
additional information | the enzyme is inhibited by Bacillus subtilis strain IJ-31 and hydrocinnamic acid, which is one of the allelochemicals produced by Bacillus subtilis strain IJ-31 | Arabidopsis thaliana | |
additional information | the enzyme is inhibited by Bacillus subtilis strain IJ-31 and hydrocinnamic acid, which is one of the allelochemicals produced by Bacillus subtilis strain IJ-31 | Capsicum annuum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Capsicum annuum | |
Fe2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin a20 + 2-oxoglutarate + O2 | Capsicum annuum | - |
gibberellin a1 + succinate + CO2 | - |
? | |
gibberellin a9 + 2-oxoglutarate + O2 | Capsicum annuum | - |
gibberellin a4 + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39103 | gene GA4 | - |
Arabidopsis thaliana Col-0 | Q39103 | gene GA4 | - |
Capsicum annuum | - |
gene GA4 | - |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein-fused enzyme from Escherichia coli by amylose affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Capsicum annuum | - |
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin a20 + 2-oxoglutarate + O2 | - |
Capsicum annuum | gibberellin a1 + succinate + CO2 | - |
? | |
gibberellin a9 + 2-oxoglutarate + O2 | - |
Capsicum annuum | gibberellin a4 + succinate + CO2 | - |
? | |
gibberellin a9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | gibberellin a4 + succinate + CO2 | - |
? | |
gibberellin a9 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana Col-0 | gibberellin a4 + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GA 3beta-hydroxylase | - |
Capsicum annuum |
GA 3beta-hydroxylase | - |
Arabidopsis thaliana |
gibberellin 3-beta-dioxygenase 1 | UniProt | Capsicum annuum |
gibberellin 3-beta-dioxygenase 1 | UniProt | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Capsicum annuum |
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Capsicum annuum |
7 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme inhibition results in growth retardation, phenotype, overview | Capsicum annuum |
additional information | treatment with either Bacillus subtilis strain IJ-31 culture extract or its allelochemicals, like hydrocinnamic acid, results in significant down-regulation of XTR9 gene expression, encoding xyloglucan endotransglucosylase/hydrolase protein 14, which is involved in plant cell elongation by cleavage of loadbearing cross-links between cellulose microfibrils, leading to wall-loosening and enabling elongation to occur, in Arabidopsis thaliana, as well as inhibition of gibberellin 3beta-hydroxxylase | Arabidopsis thaliana |
additional information | treatment with either Bacillus subtilis strain IJ-31 culture extract or its allelochemicals, like hydrocinnamic acid, results in significant inhibition of gibberellin 3beta-hydroxxylase and growth inhibition of seedlings, phenotype, overview | Capsicum annuum |