Cloned (Comment) | Organism |
---|---|
for the NTL8 complementation test, a sequence containing the NTL8 gene with its promoter is subcloned into pKGWFS7 Gateway vector, for histochemical staining an NTL8 promoter region is transcriptionally fused to the GUS-coding sequence, the fusion product transformed into Arabidopsis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transgenic plants overexpressing an active NTL8 form and T-DNA insertional ntl8-1 mutant, under standard conditions, germinate with the same rate, under high salinity stress (150 mM NaCl) the germination of the NTL8 overexpressing mutant is considerably reduced while the NTL8-deficient mutant is less sensitive, independent of absiasic acid (ABA), NTL8-deficient mutant is not impaired by gibberellin biosynthetic inhibitor paclabutrazol (PAC), gibberellin downregulates NTL8 lowering its threshold for germination | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 150 mM NaCl represses the gibberellin 3-oxidase 1 gene in the wild-type and in the NTL8-deficient mutant (NTL8 acts downstream of the gibberellin biosynthesis), repression is present but less significant in aba3-1 mutant, deficient in the stress hormone abscisic acid (independent pathway dominates), expression of GA3ox1 is recovered after a longer incubation in NaCl and leads to germination | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin 20 + 2-oxoglutarate + O2 | Arabidopsis thaliana | - |
gibberellin 1 + succinate + CO2 | - |
? | |
additional information | Arabidopsis thaliana | gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
variety Columbia (Col-0), transgenic 35S:8deltaC (overexpressing active NTL8) and ntl8-1 (lacking NTL8) mutants, aba3-1 (deficient in stress hormone abscisic acid - ABA) mutant | - |
Purification (Comment) | Organism |
---|---|
transgenic plants are ground in liquid nitrogen, total cellular extracts suspended in 1X SDS-PAGE sample loading buffer | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gibberellin 20 + 2-oxoglutarate + O2 | - |
Arabidopsis thaliana | gibberellin 1 + succinate + CO2 | - |
? | |
additional information | gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GA3 oxidase 1 | - |
Arabidopsis thaliana |
GA3ox1 | - |
Arabidopsis thaliana |
gibberellin 3 oxidase 1 | - |
Arabidopsis thaliana |