BRENDA - Enzyme Database show
show all sequences of 1.14.11.15

Separation and characterization of three 2-oxoglutarate-dependent dioxygenases from Cucurbita maxima L. endosperm involved in gibberellin biosynthesis

Lange, T.; Schweimer, A.; Ward, D.A.; Hedden, P.; Graebe, J.E.; Planta 195, 98-107 (1994)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
1 mM and more, 1.7fold stimulation
Cucurbita maxima
DTT
stimulates. Stimulation is negated when added together with DTT
Cucurbita maxima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0087
-
gibberellin a15
-
Cucurbita maxima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
stimulates. Stimulation is negated when added together with DTT. If Fe2+ is omitted, the activities are reduced by 25% compared with the maximal value reached at 0.008-0.03 mM FeSO4
Cucurbita maxima
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
gel filtration
Cucurbita maxima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
gibberellin a20 + 2-oxoglutarate + O2
Cucurbita maxima
involved in biosynthesis of gibberellin
gibberellin a1 + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cucurbita maxima
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Cucurbita maxima
Source Tissue
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Cucurbita maxima
-
seed
endosperm
Cucurbita maxima
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.065
-
-
Cucurbita maxima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gibberellin a15 + 2-oxoglutarate + O2
-
439266
Cucurbita maxima
gibberellin a37 + succinate + CO2
-
439266
Cucurbita maxima
?
gibberellin a20 + 2-oxoglutarate + O2
-
439266
Cucurbita maxima
gibberellin a1 + succinate + CO2
-
-
-
?
gibberellin a20 + 2-oxoglutarate + O2
involved in biosynthesis of gibberellin
439266
Cucurbita maxima
gibberellin a1 + succinate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000-50000, SDS-PAGE
Cucurbita maxima
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
1 mM and more, 1.7fold stimulation
Cucurbita maxima
DTT
stimulates. Stimulation is negated when added together with DTT
Cucurbita maxima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0087
-
gibberellin a15
-
Cucurbita maxima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
stimulates. Stimulation is negated when added together with DTT. If Fe2+ is omitted, the activities are reduced by 25% compared with the maximal value reached at 0.008-0.03 mM FeSO4
Cucurbita maxima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
gel filtration
Cucurbita maxima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
gibberellin a20 + 2-oxoglutarate + O2
Cucurbita maxima
involved in biosynthesis of gibberellin
gibberellin a1 + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Cucurbita maxima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Cucurbita maxima
-
seed
endosperm
Cucurbita maxima
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.065
-
-
Cucurbita maxima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
gibberellin a15 + 2-oxoglutarate + O2
-
439266
Cucurbita maxima
gibberellin a37 + succinate + CO2
-
439266
Cucurbita maxima
?
gibberellin a20 + 2-oxoglutarate + O2
-
439266
Cucurbita maxima
gibberellin a1 + succinate + CO2
-
-
-
?
gibberellin a20 + 2-oxoglutarate + O2
involved in biosynthesis of gibberellin
439266
Cucurbita maxima
gibberellin a1 + succinate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000-50000, SDS-PAGE
Cucurbita maxima
Other publictions for EC 1.14.11.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741490
Nett
Characterization of CYP115 as ...
Mesorhizobium loti, Mesorhizobium loti CJ3sym, Mesorhizobium loti MAFF303099, Mesorhizobium loti NZP2014, Mesorhizobium loti NZP2042, Mesorhizobium loti R7A, Mesorhizobium loti R88b, Mesorhizobium loti Tono, Mesorhizobium loti USDA3471, Mesorhizobium sp., Mesorhizobium sp. AA22, Mesorhizobium sp. SEMIA 3007, Mesorhizobium sp. STM4661, Mesorhizobium sp. WSM2561, Mesorhizobium sp. WSM3626, Rhizobium favelukesii, Rhizobium favelukesii OR191, Rhizobium mongolense, Rhizobium mongolense USDA1844, Sinorhizobium arboris, Sinorhizobium arboris LMG 14919, Sinorhizobium fredii, Sinorhizobium fredii CCBAU83666 AJQR, Sinorhizobium medicae, Sinorhizobium medicae WSM1369, Sinorhizobium meliloti, Sinorhizobium meliloti WSM4191, Sinorhizobium terangae, Sinorhizobium terangae WSM1721
ACS Chem. Biol.
12
912-917
2017
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33
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45
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66
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33
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66
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10
14
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741544
Otani
-
Overexpression of the gibbere ...
Torenia fournieri
Acta Hortic.
1025
23-30
2014
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743493
Chen
The maize DWARF1 encodes a gi ...
Zea mays
Plant Physiol.
166
2028-2039
2014
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1
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4
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725686
Kim
Functional mechanism of plant ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0, Capsicum annuum
J. Microbiol. Biotechnol.
22
1375-1380
2012
2
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689507
Gallego-Giraldo
Gibberellin homeostasis in tob ...
Nicotiana tabacum
Plant Cell Physiol.
49
679-690
2008
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689516
Dalmadi
Dwarf plants of diploid Medica ...
Medicago sativa
Plant Cell Rep.
27
1271-1279
2008
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700749
Kim
A membrane-bound NAC transcrip ...
Arabidopsis thaliana
Plant J.
55
77-88
2008
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700861
Urakami
Immunomodulation of gibberelli ...
Arabidopsis thaliana
Planta
228
863-873
2008
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689465
Hirano
The GID1-mediated gibberellin ...
Selaginella moellendorffii
Plant Cell
19
3058-3079
2007
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689498
Gallego-Giraldo
Flowering in tobacco needs gib ...
Nicotiana tabacum
Plant Cell Physiol.
48
615-625
2007
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676505
Mitchum
Distinct and overlapping roles ...
Arabidopsis thaliana
Plant J.
45
804-818
2006
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676607
Benschop
Long-term submergence-induced ...
Rumex palustris
Plant Physiol.
141
1644-1652
2006
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676701
Appleford
Function and transcript analys ...
Triticum aestivum
Planta
223
568-582
2006
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672812
Lester
Purification and kinetic studi ...
Arabidopsis thaliana
BMC Plant Biol.
5
19
2005
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676398
Suzuki
-
Specific expression of the gib ...
Hordeum vulgare
Plant Biotechnol.
22
195-200
2005
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676453
Solfanelli
Expression of two genes encodi ...
Phaseolus coccineus
Plant Cell Physiol.
46
1116-1124
2005
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660074
King
Gibberellin structure and func ...
Pisum sativum
Physiol. Plant.
120
287-297
2004
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10
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660239
Zhou
16,17-dihydro gibberellin A5 c ...
Arabidopsis sp.
Plant Physiol.
135
1000-1007
2004
-
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1
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3
2
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3
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1
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3
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1
1
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660243
Israelsson
Cloning and overproduction of ...
Populus tremula x Populus tremuloides
Plant Physiol.
135
221-230
2004
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660218
Ozga
Pollination-, development-, an ...
Pisum sativum
Plant Physiol.
131
1137-1146
2003
-
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6
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