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Literature summary for 1.14.11.13 extracted from

  • Lester, D.R.; Phillips, A.; Hedden, P.; Andersson, I.
    Purification and kinetic studies of recombinant gibberellin dioxygenases (2005), BMC Plant Biol., 5, 19.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000024
-
 gibberellin 20 in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36800
-
 SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum Q9XHM5
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione agarose bead affinity chromatography and gel filtration Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gibberellin 20 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 29 + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
2-oxoglutarate-dependent dioxygenase
-
 Pisum sativum
GA 2-oxidase
-
 Pisum sativum
GA 2ODD
-
 Pisum sativum
gibberellin 2-oxidase
-
 Pisum sativum